Plasma progesterone concentration during estrus cycle detected through ELIZA kit method in Kamohri goats
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2017, Vol 4, Issue 1
Abstract
Study was conducted at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam to detect estrus in time in Kamohri goat. In goats measuring plasma P4 concentration is most important to monitor luteal functions. In present study the forty adult Kamohri goats were selected and utilized in study. The goats were divided at random in to four equal treatment groups. Goats of group A received progesterone on day 0 and PGF2α along with GnRH both on day 10th, goats in group B were given PGF2α on D-0 and repeat dose of PGF2α along with GnRH on D-10th. While animals of group C were offered 50 gm of dry date fruit with 50 gm of Ajwain both daily for 10 days, whereas goats of group D received no any treatment and served as control. The goat does were treated accordingly and closely observed for estrus. The blood samples were collected from each goat to determine the estrus on the basis of plasma progesterone concentration in Kamohri goat. The serum progesterone concentration was measured by Enzyme Linkage Immuno Sorbent Assay (ELISA) kit. The results revealed that the goats of treatment groups A, B and C induced estrus 100%, irrespective of estrus synchronization treatment. However none of the goat in group D shown estrus during trial time period. The mean progesterone concentration level at day of estrus was recorded as 0.46±0.07, 0.34±0.06 and 0.36±0.05 ng/ml in treatment group A, B and C respectively and 1.38±0.04 ng/ml in control group of animal in Kamohri goat does. There were no any significant difference (P ≥0.05) was found between the treated and control group. It was concluded that the, P4 concentration provides rapid and simple method and it could be applied to monitor estrous cycles and P4 analysis can be applied as a useful tool to help to herds’ men and veterinarians in the determination of estrus so that more efficient breeding program could be developed and implemented in the flock at farm level. Keywords: GnRH, PGF2α, Enzyme Linkage Immuno Sorbent Assay (ELISA)
Authors and Affiliations
Hamzo Khan Kunbar, A. A. Memon, A. B. Kachiwal, S. A. Shaikh, H. S. Bukhari, A. Sethar
Keywords
Related Articles
- EP ID EP381272
- DOI -
- Views 111
- Downloads 0
Child Labor Contributions to the Household Productivityand Income of Rubber Farmers in Arakan Valley Complex, Philippines
Abstract:The study entitled, “ Child Labor Contributions of the Household Productivity and Income of Rubber Farmers in Arakan Valley Complex, Province of Cotabato”, was conducted to described the socio-demographic charac...
Genetic Diversity Studies of Bacteria Associated with Brycinus longipinnis from Eggua on Yewa River using Random Amplified Polymorphic DNA (RAPD) Markers
Twenty samples of Brycinus longipinnis were collected from Eggua landing site on Yewa river Ogun state, which lies on latitude 7o03ˈN and longitude 2o55ˈE. The water quality parameters (Temperature, Dissolve Oxygen and p...
Effect of dietary inclusion of browse forage (Ziziphus mucronata) in a total mixed ration on performance of Yankasa rams.
An experiment was conducted to determine the feed value of Ziziphus mucronata forage in a total mixed ration.. Sixteen Yankasa rams weighing on the average 18.19±0.26kg were divided into four groups with four animals per...
Incidence of Periparturient Complications in Surti Buffaloes
Reproductive problems in buffaloes are of significant economic concern in dairy farming. Maximizing reproductive efficiency is the goal in dairy herds for better economics return. So, present work was conducted with an a...
Adoption level of Farm Machines by Crop Farmers in Delta State, Nigeria
The study delved into the examination of the adoption level of farm machines by crops’ farmers in Delta State, Nigeria. The specific objectives were to: describe the socioeconomic characteristics of the respondents; asc...