PLASMID PROFILES OF BACILLUS GENUS ANTAGONISTIC STRAINS
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 1
Abstract
The aim of the study was to investigate plasmid profiles and to compare the results of the Gram-positive Bacillus genus bacteria plasmid isolation efficiency by various methods. Methods. Strains of B. thuringiensis and B. subtilis were used. Plasmids isolation from the cells was carried out using alkaline Kado and Liu method, Jensen method and Cross method. Results. Plasmid profiles study of B. thuringiensis and B. subtilis strains revealed that 90% of strains carried from 5 to 7 extrachromosomal genetic elements of different sizes. Conclusion. Isolated plasmids can be divided into two groups: small plasmids, about 10 kb in size and megaplasmids 100 to 200 kb. The most suitable method for the study and complete picture of B. thuringiensis or B. subtilis plasmid profiles was Jensen method adapted for bacillus megaplasmids isolation.
Authors and Affiliations
Zh. Yu. Sergieieva, V. O. Ivanytsia
Keywords
Related Articles
- EP ID EP376546
- DOI 10.18524/2307-4663.2015.1(29).48019
- Views 99
- Downloads 0
VIRION DNAs STRUCTURE OF TEMPERATE ERWINIOPHAGES 49 AND 59
The aim was to conduct a comparative study of the phages 49 and 59 virion DNAs. Methods. Properties of phage DNA were studied using the standard restriction and bioinformatic analyses. Results. The genomes of erwiniaphag...
ACTIVITY AND KINETIC PROPERTIES OF ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE IN THE INTESTINAL SULFATE-REDUCING BACTERIA
Adenosine 5′-phosphosulfate (APS) reductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 has never been well-charact...
STREPTOMYCETES BIOLOGICAL PROPERTIES AND TAXONOMICAL STRUCTURE FROM NATURAL SOILS AND TECHNOZEMS OF KRYVYI RIG AREA
Aim to select the streptomycetes from technogenic-disturbed and natural soils of Kryvyi Rig area, to establish their taxonomical structure, to carry out their identification and to assess the structure of the streptomyce...
COMPARISON OF LONG-TERM EFFECT OF TWO DYSBIOSIS MODELS IN WISTAR RATS
Aim. To compare the changes of intestinal microbial composition of two models of dysbiosis induced by ceftriaxone (Cf) or the mix of ampicillin (Amp) and metronidazole (Met). Methods. The object of the study was the chan...
STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS
Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activit...