Polihistydylowe sekwencje z motywem His-tag – ich rola i biologiczne znaczenie oddziaływania z jonami metali
Journal Title: Wiadomości Chemiczne - Year 2016, Vol 70, Issue 11
Abstract
His-tags are specific sequences containing six to nine subsequent histydyl residues and they are used commercially in immobilized metal affinity chromatography (IMAC) as molecular ‘anchors’ that bind to a metal ion (usually nickel), immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support [37, 38]. Consecutive histidines are the common denominator for both His-tags used in molecular biology and for quite remote biological phenomena – more than 2000 histidine- rich proteins (HRPs) are found in microorganisms including 60% and 82% of archaeal and bacterial species, respectively and their roles are not well characterized [73]. The physicochemical properties of histidine make it a versatile amino acid that influences protein conformation and enzymatic activity [15]. Many natural proteins with a His-tag domain are assigned to different functions, for example: most bacterial proteins, containing this motif are probably involved in the homeostasis of nickel ions [68, 76], while others, e.g. newly isolated peptides from the venom of the snake genus Atheris contain poly-histidyl-poly-glycyl sequences (pHpG) can act on the cardiovascular system by inhibiting snake venom metalloproteinases and affect its function by acting on specific receptors [58, 62]. His-rich motifs have been found also e.g. in Zn2+transporters, prion proteins, His-rich glycoproteins, transcription factors or numerous copper-binding proteins [56, 67, 84]. Binding mode and the thermodynamic properties of the system depends on the specific metal ion and the histidine sequence. Despite the wide application of the His-tag for purification of proteins, little is known about the properties of metalbinding to such tag domain. Recent experimental and theoretical studies have shown that metal ions, e.g. Cu2+can bind to various sets of imidazoles depending on the number of histidine residues that are located in His-rich sequences. The occurrence of polymorphic binding states and the formation of an α-helical structure induced by metal ion coordination suggest that proteins with a His-tag domain may serve as the dynamic site able to ‘move’ metal ions along the tag sequence [99, 100]. This might explain the frequent occurrence of such sequences in bacterial Ni2+chaperones, which transfer the metal ion between different proteins.
Authors and Affiliations
Joanna Wątły, Henryk Kozłowski
Keywords
Related Articles
- EP ID EP585059
- DOI -
- Views 127
- Downloads 0
Areny jako proleki
Nowadays, improvement of physicochemical, biopharmaceutical and pharmacokinetic properties of pharmacologically active compounds is connected with development of prodrugs. Prodrugs are defined as pharmaceutical compounds...
Elektroforeza swobodna z przepływem hydrodynamicznym : podstawy teoretyczne i rozwiązania techniczne
Free flow electrophoresis, or carrier-free electrophoresis, is a technique based upon the principle of mobility of chargeable species in the electric field. However, unlike more widespread gel electrophoresis, free flow...
Środki kontrastowe do obrazowania magnetyczno-rezonansowego na przykładzie związków kompleksowych żelaza
Nuclear magnetic resonance is already a broadly exploited phenomenon both in chemistry and medicine. Magnetic resonance imaging is a routine technique in medical diagnosis readily applied for soft tissues like brain, car...
Syntezy oliwacyny
Olivacine (1,5-methyl-6H-pyrido[4,3-b]carbazole) is an isomer of ellipticine (5,11-dimethyl-6H-pyrido[4,3-b] carbazole). Both of these heterocyclic compounds are carbazole derivatives, which exhibit broad spectrum of bio...
Biologiczna synteza nanocząstek metali
Nanotechnology has attracted a great interest in recent years due to its expected impact on many areas such as energy, medicine, electronics and space industries. One of the most important aspects in researching nanotech...