Polihistydylowe sekwencje z motywem His-tag – ich rola i biologiczne znaczenie oddziaływania z jonami metali
Journal Title: Wiadomości Chemiczne - Year 2016, Vol 70, Issue 11
Abstract
His-tags are specific sequences containing six to nine subsequent histydyl residues and they are used commercially in immobilized metal affinity chromatography (IMAC) as molecular ‘anchors’ that bind to a metal ion (usually nickel), immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support [37, 38]. Consecutive histidines are the common denominator for both His-tags used in molecular biology and for quite remote biological phenomena – more than 2000 histidine- rich proteins (HRPs) are found in microorganisms including 60% and 82% of archaeal and bacterial species, respectively and their roles are not well characterized [73]. The physicochemical properties of histidine make it a versatile amino acid that influences protein conformation and enzymatic activity [15]. Many natural proteins with a His-tag domain are assigned to different functions, for example: most bacterial proteins, containing this motif are probably involved in the homeostasis of nickel ions [68, 76], while others, e.g. newly isolated peptides from the venom of the snake genus Atheris contain poly-histidyl-poly-glycyl sequences (pHpG) can act on the cardiovascular system by inhibiting snake venom metalloproteinases and affect its function by acting on specific receptors [58, 62]. His-rich motifs have been found also e.g. in Zn2+transporters, prion proteins, His-rich glycoproteins, transcription factors or numerous copper-binding proteins [56, 67, 84]. Binding mode and the thermodynamic properties of the system depends on the specific metal ion and the histidine sequence. Despite the wide application of the His-tag for purification of proteins, little is known about the properties of metalbinding to such tag domain. Recent experimental and theoretical studies have shown that metal ions, e.g. Cu2+can bind to various sets of imidazoles depending on the number of histidine residues that are located in His-rich sequences. The occurrence of polymorphic binding states and the formation of an α-helical structure induced by metal ion coordination suggest that proteins with a His-tag domain may serve as the dynamic site able to ‘move’ metal ions along the tag sequence [99, 100]. This might explain the frequent occurrence of such sequences in bacterial Ni2+chaperones, which transfer the metal ion between different proteins.
Authors and Affiliations
Joanna Wątły, Henryk Kozłowski
Keywords
Related Articles
- EP ID EP585059
- DOI -
- Views 129
- Downloads 0
Prolina : pospolity aminokwas wyjątkowy katalizator. Część IV, Reakcja Michaela
In recent years there has been a dynamic development of asymmetric synthesis. Groups of researchers, particularly the one led by Benjamin List and Carlos Barbas, carried out a number of reactions and showed the effective...
Znaczenie mikroporowatych materiałów metaloorganicznych (MOF ) dla potrzeb magazynowania wodoru
Currently, the metal-organic frameworks (MOF s) are considered among the most promising materials for hydrogen storage. In this paper, the properties of MOF s that are particularly important for application purposes are...
Fotoreaktwne poliakrylanowe kleje samoprzylepne
Pressure-sensitive adhesives (PSAs) are non-metallic materials used to bond other materials, mainly on their surfaces through adhesion and cohesion. Adhesion and cohesion are phenomena, which may be described thermodynam...
Metody syntezy 2,2'-bipirolidyny
Vicinal diamine derivatives form an interesting and significant class of organic compounds. They play an essential role as chelating agents and also as ligands for asymmetric synthesis. They are used in synthesis of biol...
Estry kwasów tłuszczowych glicydolu oraz estry kwasów tłuszczowych mono-3-chloropropan-1,2-diolu : nowe zanieczyszczenia w olejach jadalnych
The aim of the review was to characterize and describe the physicochemical properties and methods for the determination of two types of compounds: 3-monochloropropane- 1,2-diol fatty acids esters (3-MCPD esters) and glyc...