Polysaccharide extracts of Cirsium japonicum protect rat H9c2 myocardial cells from oxidative stress induced by hydrogen peroxide
Journal Title: Traditional Medicine Research - Year 2018, Vol 3, Issue 3
Abstract
Daji (Cirsium japonicum) has been applied against gastric disorders, lung diseases, and cardiovascular problems in the traditional Chinese medicinal system. The present study was to investigate the protective effects of Daji (Cirsium japonicum) polysaccharide extracts (CJP) against hydrogen peroxide (H2O2) shock in rat H9c2 myocardial cells. First, CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquid chromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cell injury model. The 3- (4,5- dimethylthiazol- 2-yl)-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatment significantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2 was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. In addition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP on myocardial cells. Therefore, we conclude that polysaccharide extracts of Daji (Cirsium japonicum) protect rat H9c2 myocardial cells from oxidative stress induced by H2O2.
Authors and Affiliations
Zheng-Bo Tao, Li-Yan Xiong, Li-Hui Wang, Chuan Zhang
Keywords
Related Articles
- EP ID EP45232
- DOI 10.12032/TMR201811072
- Views 243
- Downloads 0
Is Colla Corii Asini just boiled donkey skin?
The preparation of Chinese medicine, exploration of the bioactive component, and underlying mechanism of traditional Chinese medicine (TCM) have always been regarded as a research hotspot and have attracted increasing at...
Antitumor applications of nano-traditional Chinese medicine
An article by Deng et al. [1] that was first published in ACS Nano in 2019 revealed that nanoparticles extracted from cuttlefish ink (CINPs) could inhibit tumor growth by synergizing immunotherapy and photothermal therap...
Effect of Jianpi Jiedu Recipe on angiogenesis and the PTEN/PI3K/AKT signaling pathway in the course of Helicobacter pylori-induced gastric cancer in C57BL/6 mice
Objective: To reveal the effect of Jianpi Jiedu recipe (JPJDR) on angiogenesis and the PTEN (Phosphatase and tensin homolog deleted on chromosome ten)/PI3K/AKT signaling pathway in the course of H. pylori infection-induc...
Medical workers in traditional medicine who sacrificed their lives in the battle against COVID-19 deserve to be remembered forever
China held a national day of mourning on April 4, 2020 to commemorate 3,335 victims of the COVID-19 outbreak, including medical workers who sacrificed their lives in the battle against the epidemic. April 4 was also Tomb...
A multi-center randomized controlled clinical trial of three-step acupuncture and cupping therapy for cervicogenic headache
Objective: To assess the clinical efficacy and safety of three-step acupuncture and cupping therapy for cervicogenic headache (CEH). Methods: A randomized, single-blind, multi-center, parallel controlled clinical trial w...