Possibility of application of molecular markers for the assessment of genetic diversity of oilseed rape breeding lines
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2010, Vol 31, Issue 2
Abstract
The knowledge of general and specific combining ability, information about pedigree as well as genetic distance help in selection of parental forms for F1 hybrids. The relationship of molecular markers diversity of parental lines of F1 hybrids with heterosis effect and yielding ability of hybrids has been examined in numerous studies for different crop plants. The aim of this study was: to assess genetic distance (GD) based on RAPD and AFLP markers, to compare and to evaluate the relationship of GD estimated by two molecular techniques using 178 RAPD and 259 AFLP markers and also using the lower number of molecular markers. Genetic distance of 7 oilseed rape breeding lines (parental lines of CMS ogura hybrids) was estimated using 23 AFLP primer combinations and 64 RAPD primers. AFLP primer combinations generated 1026 bands, of which 25.2% were polymorphic and RAPD primers generated 361 bands, of which 49.3% were polymorphic. The UPGMA (Unweighted Pair Group Method of Arithmetic Mean) method was used to construct dendrograms based on the Nei and Li (1979) diversity coefficient. Genetic distance was computed separately for RAPD, for AFLP markers and for two marker types together and also for different numbers of markers. The genetic distance between investigated CMS ogura and restorer lines ranged from 0.76 to 0.88 and from 0.22 to 0.29 for CMS ogura and maintainers lines. The obtained results revealed statistically significant correlation between GD estimated by 178 RAPD markers (64 primers) and 259 AFLP markers (23 primer combinations) but also for lower number of the RAPD and AFLP markers. Dendrograms based on GD values evaluated on the basis of RAPD markers, AFLP markers, the both types of markers as well as using different number of the markers placed the investigated genotypes in similar arrangement.
Authors and Affiliations
Alina Liersch, Krystyna Krótka, Iwona Bartkowiak-Broda
Keywords
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