PRE-MARITAL SCREENING TESTS OF β-THALASSEMIA TRAIT IN DAKSHINA KANNADA POPULATION OF KARNATAKA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 2
Abstract
Objective: β-Thalassemia is one of the familiar single gene disorders which passes from parents to offspring. The prevalence of β-thalassemia trait varies from 1-14% in different regions of India. Every year almost 9000 β-thalassemic major children are being born in the Indian sub-continent. In the present study, the prevalence of β-thalassemia trait was checked and some screening tests were performed to detect it among the Dakshina Kannada population of Karnataka.Methods: A total of 800 youngsters were selected for the study, males being above 21 y and females above 18 y. Two ml of blood was drawn and collected in K2 EDTA bottles and complete hemogram was immediately checked. Samples which have Mean Corpuscular Volume (MCV)<80 fico litres(fl) were selected for the study. Five discriminant functions were calculated. NESTROFT (Naked Eye Single Tube Red Cell Osmotic Fragility Test) was performed in all the samples. The samples which show positive for NESTROFT and at least 2 discriminant functions were further checked for HbA2 level using cellulose acetate electrophoresis to confirm the β-thalassemia trait. A comparison was made with the normal samples which have MCV ˃ 80fl.Results: Prevalence of β-thalassemia trait was found to be 5.125 % in this population. The obtained values were analyzed using unpaired Student’s‘t’ test using GraphPad prism (Version-3.0). Samples of β-thalassemia trait have significant changes in the white blood corpuscles (WBC p=0.1266), red blood corpuscles (RBC p=0.0130), hemoglobin (Hb p<0.0001), hematocrit (HCT p<0.0001), MCV(p<0.0001), mean corpuscular hemoglobin (MCH p<0.0001), mean corpuscular hemoglobin concentration (MCHC p<0.0001), platelets (PLT p=0.0005), HbA2(p<0.0001) compared to normal controls.Conclusion: The present study shows that the people with β-thalassemia trait have a significant variation in complete hemogram compared to normal; NESTROFT and discriminant functions can be used for the screening of β-thalassemia trait in the population.
Authors and Affiliations
Suresh Babu T. V. , Saritha . , Ashwini S. , Manjula Shantaram
Keywords
Related Articles
- EP ID EP575854
- DOI 10.22159/ijpps.2017v9i2.16320
- Views 76
- Downloads 0
EFFECT OF SUGNIL, A TRADITIONAL ANTIDIABETIC HERBAL FORMULATION ON THE EXPRESSION OF TRANSCRIPTION FACTORS IN STREPTOZOTOCIN DIABETIC RATS.
Objective: A number of large randomized clinical trials demonstrated that lipid lowering successfully reduced cardiovascular events and death in patients with diabetes. Recent studies have reported that multiple transcri...
COFFEE: A HEALTH FUEL-BLOT POPULAR DRINKING
Now, the days begin with cups of coffee worldwide. Caffeine is the main component of coffee, which is vastly consumed as a psychoactive agent, and in varieties of dietary supplements. Day by day coffee and caffeinated-co...
ANTI-INFLAMMATORY STUDIES IN RELATION TO CHEMICAL EVALUATION ON TWO SPECIES OF AN INDIGENOUS DRUG ‘BARLERIA’
Objective: The present study was aimed to assess the anti-inflammatory activity profile of Barleria spp. viz., B. prionitis Linn. and B. cristata Linn. against different models of inflammation in female rats.Methods: Sev...
FEASIBILITY OF AN INTENSIVE CONTROL INSULIN-NUTRITION GLUCOSE MODEL ‘ICING’ WITH MALAYSIAN CRITICALLY-ILL PATIENT
A clinically verified patient-specific glucose-insulin metabolic model known as ICING is used to account for time-varying insulin sensitivity. ICING was developed and validated from critically-ill patients with various m...
IDENTIFICATION OF POTENT BROMODOMAIN4 (BRD4) INHIBITORS BY ENERGY-PHARMACOPHORE BASED VIRTUAL SCREENING TO TARGET BRD4-NUT MIDLINE CARCINOMA
Objective: Protein-protein interactions (PPI’s) have been used as a target in various diseases. One such major role of PPI’s comes when the protein Bromodomain4 (BRD4) reads the epigenetic changes in the histones and...