Preclinical evaluation of antidiabetic activity of poly herbal plant extract in streptozotocin induced diabetic rats
Journal Title: UNKNOWN - Year 2016, Vol 5, Issue 2
Abstract
Objective: To study and compare the effect of Poly herbal plant extract (PHPE) with Glibenclamide (GL) on various parameters in Streptozotocin (STZ) induced diabetic rats. Methods: Diabetes was induced by combining High Fat-diet and injecting low dose Streptozotocin (35 mg/kg body wt.) to Sprague-dawley rats. Diabetic rats were treated with chloroform leaf extract of Azadirachta indica, aqueous leaf extract of Bougainvillea spectabilis and ethanolic seed extract of Trigonella foenum graecum combined in ratio of 1:2:3 respectively at dose of 600 mg/kg body weight by oral gavaging for 28 days. The results were compared with standard anti diabetic drug Glibenclamide given in dose of 500 µg/kg body weight. Results: Increase in body weight of both PHPE and GL treated diabetic rats was found to be statistically significant (p<0.05) compared to diabetic control rats. Decline in FBG levels of both PHPE and GL treated diabetic rats were found to be highly significant statistically (p<0.001) when compared to diabetic control rats at the end of study. Total Cholesterol (TC) and Triglycerides level in diabetic rats treated with PHPE were found to be highly statistically significant (p<0.001) compared to diabetic control rats. Pancreas of PHPE treated diabetic rats revealed partial restoration in size and number of islet of langerhans. Reduction in widening between acinar and islet cells noted. Glibenclamide treated diabetic rats showed much more improvement in pancreatic cell architecture by returning to its normal structure and size. Conclusion: In present study PHPE has shown to decrease elevated FBG level and improve in body weight at the end of study in diabetic rats which can be suggested due to modification in carbohydrate metabolic pathways, stimulation of insulin production by the pancreas, increased peripheral utilization of glucose in the cells and regeneration of β-cells of the pancreas.
Authors and Affiliations
PP Gupta, J Haider, RP Yadav, U Pal
Keywords
Related Articles
- EP ID EP455399
- DOI -
- Views 83
- Downloads 0
Quantifying phytophenols in Andrographis paniculata and Withania somnifera leaf extracts
Andrographis paniculata (Nilavembu) and Withania somnifera (Ashwagandha) are some of the commonly available herbs, used for treating a wide range of ailments in man and animals. Both the plants possesses a wide spectrum...
A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi
Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Gin...
Effect of Seabuckthorn leaves on Antioxidant and Microsomal Enzymes in poultry birds
In vivo studies on broiler birds were carried out to evaluate effect of aflatoxin and seabuckthorn leaves on microsomal enzyme system, antioxidant enzymes and biochemical parameters i.e. serum triglyceride, total plasma...
Baillonella toxisperma improves sexual performance and protects against stress-induced reproductive dysfunction in male Wistar rats
Baillonella toxisperma Pierre (Sapotaceae) is a medicinal plant widely used in Central Africa against several diseases including erectile dysfunction and male infertility. However, no study dealing with these male reprod...
Resistance modulation studies of vernolide from Vernonia colorata (Drake) on ciprofloxacin, amoxicillin, tetracycline and erythromycin
Resistance modulatory activities of natural products on traditional antibiotics have gained scientific interest in recent years. This is aimed at maximizing antimicrobial potency with major strides in curbing microbial r...