PRELIMINARY CHARACTERIZATION, ANTIOXIDANT ACTIVITIES AND ULCER CURATIVE EFFECT OF OPUNTIA FICUS INDICA F. INERMIS ROOTS POLYSACCHARIDES IN RATS
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 4
Abstract
The present study was undertaken to investigate the in vitro antioxidant activity and the in vivo curative effect of Opuntia ficus indica f. inermis polysaccharides (OFIP) against ethanol- induced ulcer in rats. According to gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy studies OFIP was an heteropolysaccharide composed of rhamnose, arabinose, fucose, mannose, glucose and galactose at the molar ratio of 4.91; 4.94; 3.87; 32.51; 7.8; 7.1. In vitro tested for their potential antioxidant activity OFIP exhibited high radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) when compared with butylated hydroxyanisole (BHA) activity. Whereas OFIP hydroxyl radical scavenging activity, reducing power and the lipid peroxidation inhibitory effect appeared low but not weak when compared with the BHA and vitamin C activities. In vivo the treatment with OFIP at oral doses 100, 200, and 400 mg/kg b.w., was found to provide a dose-dependent protection against ethanol-induced gastric ulcer by reducing the gastric juice output, enhancing the healing rate and increasing the mucus production. The antiulcerogenic activity of OMFE might be due to a possible synergistic antioxidant, anti-excretory and healing mechanisms.
Authors and Affiliations
Hichem Alimi*| Research unit of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, University of Gafsa, 2112 Gafsa, Tunisia.-Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia., Zouhour Bouoni| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Anwer Feriani| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Najla Hfaeidh| Laboratory of Animal Ecophysiology, Faculty of Science of Sfax, University of Sfax, 3018 Sfax, Tunisia., Mohsen Sakly| Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia., Khémais Ben Rhouma| Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 7021 Jarzouna, Bizerte, Tunisia.
Keywords
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