Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii
Journal Title: Journal of Microbiology & Microbial Technology - Year 2016, Vol 1, Issue 1
Abstract
Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highest reducing activity in presence of 1% (w/v) hexavalent Chromium. NADPH at a conentration of 2 mM was found to act as electron donor in the reduction reaction. The intracellular chromate reductase was found to have an optimum pH and temperature of 7.4 and 70 °C respectively. The enhancement of reductase activity in presence of exogenous thiols indicated the presence of thiols at active site.
Authors and Affiliations
Rina Rani Ray
Keywords
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- EP ID EP246370
- DOI 10.13188/2474-4530.1000003
- Views 119
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Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii
Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highe...