Preliminary study of the effect of PD-L1 on the biological behavior of vascular endothelial cells in keloids
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 7
Abstract
Objective To clarify the difference in the expression of PD-L1 in vascular endothelial cells of keloids and normal skin tissues, and to clarify the regulatory effect of PD-L1 on vascular biological behavior through in vitro function experiment. Methods Clinical samples of keloid were collected, its vascular structure was observed by HE staining, the expression of PD-L1 was detected by Western blotting and PCR, and the expression level of PD-L1 in tissues was observed by immunohistochemistry and immunofluorescence staining. Immunofluorescence was used to determine the position relationship between inflammatory cells and vascular endothelial cells in keloid tissues. The primary extracted T cells were co-cultured with PD-L1 overexpressed vascular endothelial cells, and the functional status of vascular endothelial cells was detected by lumen formation, Transwell cell migration assay and Ki-67 staining. Results Compared with normal skin tissues, the vascular structure and function in keloid tissues were disordered and abnormal, and the expression of PD-L1 in vascular endothelial cells was significantly decreased with regional differences. After co-culture with T cells, the lumen formation of vascular endothelial cells in the overexpressed PD-L1 group increased, the migration efficiency increased, and the proliferation was vigorous. Conclusion The expression of PD-L1 in vascular endothelial cells of keloid tissues decreased with regional differences. With the participation of T cells, the high expression of PD-L1 in vascular endothelial cells can promote the proliferation, migration and lumen formation of vascular endothelial cells, which may increase the risk of keloid formation.
Authors and Affiliations
DONG Ruina, LYU Yajie, WANG Bingkun, CAO Tianyu, ZHAI Da, ZHANG Yanguo
Keywords
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