Preparation and Characteristic of Dextran-BSA Antibody and Establishment of it’s Elisa Immunoassay
Journal Title: International Journal of Food Science, Nutrition and Dietetics (IJFS) - Year 2014, Vol 3, Issue 6
Abstract
The enzyme linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextran–BSA antigens were prepared by Reductive amination method, and were confirmed by SDS-PAGE and free amino detection. The effects of coupled reaction conditions such as different oxidation degree of dextran, the reaction time were investigated and the immunity of the resulting dextranBSA neoglycoprotein antigens were evaluated through the interaction with standard dextran antibody. The immunogen was immunized with white rabbits to obtained polyclonal antibody respectively. A general and broad class-specific Elisa detection method was developed according to Elisa theory. The method was put to use for quantitative analysis of dextran in practical saccharose samples.
Authors and Affiliations
Xie Zhen-ming
Keywords
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- EP ID EP200586
- DOI 10.19070/2326-3350-1400024
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