Preparation of psyllium husk powder based microporous composite scaffolds for tissue engineering
Journal Title: Engineering of Biomaterials / Inżynieria Biomateriałów - Year 2018, Vol 21, Issue 147
Abstract
This study demonstrates the comparison in the method of fabrication and thus evaluates the potential of psyllium husk powder and gelatin-based composite microporous scaffolds for tissue engineering applications. The scaffold is being prepared in three different ratios of 50:50, 75:25 and 100 (w/w of psyllium husk powder and gelatin, respectively) by employing a suitable cross-linking agent, EDC-NHS, followed drying. We have demonstrated the use and outcomes of two different methods of scaffold drying, i.e., vacuum desiccation along with liquid nitrogen dip and lyophilization. It was concluded from the SEM micrographs that the scaffolds dried under vacuum accompanied with liquid nitrogen exposure exhibited less porous architecture when compared to those prepared using a lyophilizer, that resulted in pores in the range of 60-110 μm. Scaffolds fabricated using the former technique lost porosity and sponge-like characteristics of a scaffold. In spite of the above fact, water retaining capacity and stability in the cell culture of such scaffolds is significant, nearly 40-50% of its initial dry weight. Cell culture experiments support the potential of the scaffolds prepared from different methods of fabrication for its cytocompatibility and suitability for cell growth and proliferation for a substantial duration. Erosion in the porous design of the scaffolds was observed after 14 days via SEM micrographs. It was inferred that freeze-drying is a better technique than vacuum desiccation for scaffold preparation. The present investigation has been conducted keeping in mind the importance of drying a scaffold. Scaffold drying is a necessary step to increase its shelf-life, makes it easy to transport and much importantly, controlling the pore size of the scaffold.
Authors and Affiliations
S. Poddar, P. S. Agarwal, S. K. Mahto
Keywords
Related Articles
- EP ID EP553974
- DOI -
- Views 74
- Downloads 0
Improvement of short Ca-P whiskers/polylactide composites by surface modification with lauric acid
Polymer-ceramic composites are widely used in regenerative medicine, especially in orthopedics, as there are many methods of modifying their properties depending on the intended use. One of them is filling the polymer ma...
Tribological properties of resorbable polylactide-based biomaterials
The objective of this study was the observation and evaluation of tribological characteristics and behavior of biocomposites with polymer matrix under different friction conditions. Three types of polylactide composites...
Ocena biologiczna in vitro nowych biomateriałów stomatologicznych
Ponieważ znane jest działanie bakteriobójcze srebra i złota, podjęto próbę wytworzenia nowej grupy biomateriałów bakteriobójczych do stosowania w stomatologii odtwórczej, zawierających cząstki nanosrebra i nanozłota. Cel...
Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged and uncharged ferroelectric crystal surfaces
The cell-material interaction is significantly influenced by the physicochemical properties of the material surface, including its electrical charge. In this study, the effect of the surface polarity of ferroelectric LiN...
Interactions of carbon nanoparticles from packagings with components of food, drugs and biologically active molecules - a review
Nanomaterials are very important in the field of packaging of food, medicines and dietary supplements. Modern packagings often contain nanoparticles that provide them new feature - nanoparticles are used to activate main...