Prevalence of ESβL types TEM, SHV, CTX in isolates of Salmonella, Citrobacter and Enterobacter spp. from patients feaces in Tabriz
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 9
Abstract
Extended-spectrum β-lactamase-producing bacteria are a worldwide healthcare problem exacerbated by antibiotic use and transmission of resistant bacteria by carrier. This study analyzed stool carriage of ESβL producing Salmonella, Citrobacter and Enterobacter spp.in the outpatients and inpatients in order to assessment entrance of these bacteria into hospitals. During the period from April 2015 to July 2016 stool samples were obtained from 200 out patients at beginning of admission and 200 inpatients after 48 hours hospitalization. Specimens were cultured in CTX-MacConkey agar and selenite F broth for salmonella enrichment. All cefotaxim resistant bacteria were identified by biochemical tests and ESBL-producing bacteria were detected using double-disk synergy test (DDST). Susceptibility of ESBL-producing isolates was also determined by disk diffusion method. TEM, SHV and CTX genes were investigated by PCR. Of the 400 stool samples tested, 9 (2.25%) ESBL producing bacteria including 2 (1%) from outpatients and 7 (3.5%) from inpatients were isolated. Citrobacter freundii was the predominant ESBLproducing organism 4(1%). No Salmonella typhi was detected but two (0.5%) Salmonella typhimurium from in patients were isolated. Overall, imipenem and amikacin were the antibiotics most active against the ESBL producing organisms. blaCTX-M, blaTEM, blaSHV, were noticed in 75%, 50%, and 25%, of citrobacter isolates, respectively. Patients are one of the most important carriers of ESBLs to hospitals. In addition, our findings suggest the concurrent society spread of different ESBL genotypes, not a development of special ESBL genes.
Authors and Affiliations
Mohammad Taghi Akhi| Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Tabriz University of Medical Sciences, Tabriz, International Branch (Aras), Tabriz, Iran, Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran, Elmira Najafi Nasab| Tabriz University of Medical Sciences, Tabriz, International Branch (Aras), Tabriz, Iran, Corresponding e-mail: elmira_najafinasab@yahoo.com, Reza Ghotaslou| Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran, Tahere Pirzadeh| Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran, Mohammad Asgharzadeh| Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Pourya Gholizadeh| Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran, Aylin Esmailkhani| Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran, Vida Sorayaei Somehsaraei| Tabriz University of Medical Sciences, Tabriz, International Branch (Aras), Tabriz, Iran, Aydin Akhi| Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords
Related Articles
- EP ID EP12033
- DOI -
- Views 287
- Downloads 11
Barriers professional competence and its relationship with job satisfaction of nurses' moral distress and pre-hospital emergency city of Bam and Jiroft in 1393
In order to "protect the health of people" Several organizations have been founded and given its role in saving lives when seconds play, is formed Medical Center Emergency Management Whose duty is satisfactory service i...
Comparison of Heavy- and Light-Weight Composite Mesh in Pain Reduction Among Patients with Inguinal Hernia: A 1-Year Randomized Control Trial
Background: Inguinal hernia is commonly addressed complication among men and women. Surgical mesh repair is widely used as a treatment. Postoperative chronic pain is a major complication and concern, as it restricts the...
INCIDENCE AND LOCATION OF ZYGOMATICOFACIAL FORAMEN IN ADULT HUMAN SKULLS
This study was to investigate the morphology, topographic anatomy and variations of Zygomaticofacial foramen (ZFF). Frequency variations and Location/distance of ZFF from surrounding standard landmarks were evaluated in...
CLINICAL PATTERN AND EFFECT OF CO-MORBIDITIES IN THE ETIOPATHOGENESIS OF INCISIONAL HERNIAS
Background: Incisional hernia is a common iatrogenic complication of abdominal surgery and is a cause of unwanted morbidity. The study was reported for the first time from Republic of Mauritius. Aims & Objectives: The...
A STUDY ON ASSOCIATION OF SMOKING AND GASTRIC CARCINOMA IN THE RESIDENTS OF WEST BENGAL
Objectives: The aim of the study is to know the association of tobacco intake in the form of smoking and chewing with gastric carcinoma in West Bengal. Materials and methods: Total 28860 patients (smokers and tobacco c...