Prevalence of Glucose -6- Phosphate Dehydrogenase (G-6-PD) Deficiency in Sokoto: Liver Function and Oxidative Stress Biomarkers in Deficient Individual
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 13, Issue 11
Abstract
Background: Glucose-6-phosphate dehydrogenase (G6PD) is a key enzyme in the pentose phosphate pathway (PPP) and plays an essential role in the oxidative stress response by producing Nicotinamide adenine dinucleotide phosphate (NADPH), the main intracellular reductant. Deficient individuals suffer from mild chronic haemolytic episode which could be exacerbated on exposure to oxidant drugs. Aim: The aim of the study was to determine the prevalence of G-6-PD deficiency in Sokoto, assess liver function, lipid peroxidation and antioxidants status in G-6-PD deficient individuals. Place and Duration of Study: The study was undertaken at the Department of Chemical Pathology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria, between February and April 2015. Methods: G-6-PD screening in 1000 individuals (603 males and 397 females) using Methaemoglobin Reduction Method was carried out, liver function and oxidative stress biomarkers were then evaluated in 60 deficient individuals (30 males and 30 females) and 60 individuals with normal G-6-PD status as controls using standard techniques. Results: 376 (37.6%) subjects were found to be G-6-PD deficient, 128 (12.8%) of the males and 248 (24.8%) of the females screened were deficient. G-6-PD deficient individuals have significantly low (p<0.05) total protein (TP), aspartate transaminase (AST) and alkaline phosphatase activities when compared to control group but the decreases were within the reference range, while albumin (Alb), total bilirubin (TB) and conjugated bilirubin (CB), alanine transaminase (ALT) and alkaline phosphatase (ALP) values showed no significant difference (p > 0.05). Significantly high (p<0.001) malondialdehyde (MDA) and low total antioxidant potential (TAP) values were obtained in G-6-PD deficient individuals compared to controls. Conclusion: The prevalence of G-6-PD deficiency in Sokoto is high, hence screening for G-6-PD deficiency before administration of oxidant drugs in G-6-PD deficient subjects may be necessary. G-6-PD deficient individuals may also be at the risk of developing oxidative stress induced diseases.
Authors and Affiliations
Taofeeq Oduola, Ismaila Jelani, Debayo Moshood Bolarin, Mohammed Alhaji Ndakotsu, Muhammad Kabiru Dallatu
Keywords
Related Articles
- EP ID EP340724
- DOI 10.9734/BJMMR/2016/24149
- Views 71
- Downloads 0
Lipoprotein-associated Phospholipase A2, Lp-PLA2, and Age, are Predictors for Future Cardiovascular Events in Acute Coronary Syndrome Patients
Aims: We investigated whether Lp-PLA2 activity of blood samples collected 24 h after hospital admission could serve as a predictor of future cardiovascular events in Vietnamese patients with acute coronary syndrome (ACS)...
Dietary Patterns and Weight Loss in New-onset Type 2 Diabetes Mellitus: A Sub-analysis of the St Carlos Study: A 3-year, Randomized, Clinic-based, Interventional Study
Objective: To assess lifestyle patterns associated with weight loss in newly-diagnosed type 2 diabetic patients (T2DM) in the St Carlos Study. Design: A 3-year, randomized, interventional study with three parallel groups...
Etiologic Evaluation of 1012 Patients Admitted with Thrombocytopenia
Introduction: Thrombocytopenia is the situation where the number of thrombocytes is less than 150.000/ µL. This may result due to decreased thrombocyte production, increase in the destruction of thrombocytes and differen...
Evaluation of Simple and Cost-Effective DNA Preparation and Subsequent PCR Amplification for Clinically Relevant Mycobacteria
Aim: Slow growth rate in culture renders the traditional isolation, identification, and drug susceptibility testing of clinically important mycobacteria inadequate when there is an urgent need for a precise diagnosis in...
Monocyte Chemoattractant Protien-1 (MCP-1) and Atherosclerosis in End Stage Renal Disease Patients
Background: Emerging evidences suggest that inflammation is involved in the pathogenesis of cardiovascular diseases. Monocyte chemoattractant protein-1 (MCP-1) has a central role in atherogenesis. The study was performed...