Production and characterization of antimicrobials from isolate Pantoea agglomerans of Medicago sativa plant rhizosphere soil
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 2
Abstract
Due to rise in drug resistance among pathogens, there is always an urge to look for new drug alternatives. So in this study we aimed to identify the unexplored rhizosphere microflora of alfalfa plant for new antimicrobials. With initial screening for isolates from rhizosphere region for antibacterial activity against selected bacterial pathogens, the isolate AL10 had better activity selected for this study. The isolate mass was cultured and secondary metabolites were extracted using ethyl acetate and subjected to FTIR and GC-MS analysis. Based on functional diversity analysis, the isolate subjected to anti-bacterial activity revealed significant activity against Streptococcus pneumonia, Klebsiella, S. aureus with zone of inhibition in the range of18-20 mm. Based on GC-MS analysis report ten compounds were identified and 1-Octadecane and 1-nonadecanol were found to be responsible for bio-activity. FT-IR results showed that N-H stretching functional group was dominantly present in the extract. Molecular identification of the isolate by 16S rRNA sequencing showed the isolate as Pantoea agglomerans. The results showed that the isolate P.agglomerans, gram negative bacteria had wide antibacterial activity due to 1-Octadecane and 1-nonadecanol. Though Alfalfa plant has been described for various biological activities, this is a first report on rhizosphere region of plant reporting for antibacterial potential microbes.
Authors and Affiliations
Nisha M Nair, R. Kanthasamy, S. Iruthaya Kalai Selvam, S. Ramalakshmi
Keywords
Related Articles
- EP ID EP588655
- DOI 10.31018/jans.v11i2.2031
- Views 85
- Downloads 0
Estimates of genetic components and regression analysis for grain yield and various morphological traits in bread wheat (Triticum aestivum L.)
The present study was conducted to estimate the gentic components and regression analysis for grain yield and various morphological traits in bread wheat involving 10 parents and their 45 F1s (half diallel) during 2012-...
Applications of submerged fermentation for biodegradation and decolourisation of melanoidins by an isolate Alcaligenes denitrificans SAG5
In laboratory conditions a bacterium was isolated, which was identified as Alcaligenes denitrificans SAG 5. . The optimum decolourisation (72.6%) of melanoidin was achieved at pH 7.5 and temperature 37 °C within 4-6 days...
Effect of different storage conditions and seed treatments on seed viability in soybean [Glycine max (L.) Merr.]
The present investigation was carried out in laboratory of the Department of Seed Science and Technolo-gy, College of Agriculture, Junagadh Agricultural University, Junagadh from the April 2013 to April 2015, wherein two...
Response of walnut (Juglans regia. L.) leaf aqueous extracts, on seed germination and seedling growth of wheat (Triticum aestivum L.) in agri-silvi system of Uttarakhand
Effect of different doses of aqueous extracts of walnut leaf was studied on germinating seeds and early seedling growth of wheat variety (cv. VL-616) recommended for hills under West Himalayan agri-silvi system. Seven tr...
Genetic variability study in F2 population of tetraploid dicoccum wheat crosses
The aim of the present investigation was to estimate the genetic variability parameters in Wheat (Triticum dicoccum) F2 population of the cross DDK1025 X ML-1. The traits involved in study were grain yield, threshability...