Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate
Journal Title: Biotechnology Journal International - Year 2016, Vol 13, Issue 1
Abstract
The production of cellulase from Aspergillus flavus isolated from Brewers’ Spent Grain (BSG) undergoing bio-deterioration was carried out using solid state fermentation method. Out of a total of four fungal isolates with cellulase producing potentials, Aspergillus flavus with a clearance zone of 5.0cm was the best hyper-producer and was selected for optimization and cellulase production. Two different enzyme production media containing either yeast extract or potassium nitrate (KNO3) served as sources of nitrogen to the Aspergillus flavus. The crude activity of the potassium nitrate (KNO3) option (28,170 Units) was significantly higher than the crude cellulase from yeast extract option (18,600 Units). Ammonium sulphate precipitation at 60%, 80%, and 100% had cellulase activities 14600 units, 4400 unit, and 2800uits in yeast extract amended experimental option. In addition, ammonium sulphate precipitation at 60%, 80%, and 100% gave cellulase activities of 21130 units, 1700 units, and 1110 units, respectively in the KNO3 amended experimental option. Thus, 60% ammonium sulphate precipitation gave the best cellulase activity. The optimum pH of Aspergillus flavus cellulase was 6.0, while the optimum temperatures were between 50°C to 60°C. Tolerance to some alkali and alkaline–earth metals was also established, and probable co-factor behaviour of manganese ion to this cellulase was also established. Good government policies that support high import duties on foreign enzymes, emphasis on local production are advocated for in order to stamp out the economic leakages accruing through enzyme importation into Nigeria.
Authors and Affiliations
F. A. Orji, E. N. Dike, A. K. Lawal, Y. L. Suberu, B. Ita, G. N. Elemo
Keywords
Related Articles
- EP ID EP237866
- DOI 10.9734/BBJ/2016/24882
- Views 106
- Downloads 0
In vivo and In vitro spasmolytic Effect of Ficus sur Forssk Ethanol Leaf Extract on the Gastrointestinal Tract
Aim: Sequel to the report that Ficus sur ethanol leaves extract (FELE) has anti-diarrheal property and has been used in some parts of Nigeria for the treatment of the disease, this work examined the effect of Ficus sur e...
Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo
Aim: The hypothalamic hormone, growth hormone-releasing hormone, is the principal stimulator of pituitary growth hormone (GH) synthesis and secretion. GHRH and its receptor (GHRHR) provide important functions in the regu...
Integration of Reticuloendotheliosis Virus in Most of Tanzanian Fowl Pox Virus Isolates is not attributed to Imported Commercial Fowl Pox Vaccines
Aim: To investigate integration of reticuloendotheliosis virus (REV) in the Tanzanian fowlpox virus (FWPV) field isolates, and the imported commercial fowl pox vaccines currently used in the country. Study Design: Exper...
Molecular Relatedness and Morpho-Agronomic Characteristics of Congolese Accessions of Cassava (Manihot esculenta Crantz) for Breeding Purposes
Cassava serves as primary staple food of millions of people in the tropics and subtropics, and is used as a carbohydrate source in animal feed. Knowledge of agro-morphological characteristics and genetic relatedness is e...
Histopathological Alterations Induced by Naja naja Crude Venom on Renal, Pulmonary and Intestinal Tissues of Mice Model
Aim: Snake bite causes a significant number of mortality and morbidity throughout the world. So, the current study was carried out to estimate the extinct of damage caused by intraperitoneal introduction of cobra venom o...