Protein aggregation and bioprocessing
Journal Title: The AAPS Journal - Year 2006, Vol 8, Issue 3
Abstract
Protein aggregation is a common issue encountered during manufacture of biotherapeutics. It is possible to influence the amount of aggregate produced during the cell culture and purification process by carefully controlling the environment (eg, media components) and implementing appro-priate strategies to minimize the extent of aggregation. Steps to remove aggregates have been successfully used at a manufacturing scale. Care should be taken when developing a process to monitor the compatibility of the equipment and process with the protein to ensure that potential aggregation is minimized.
Authors and Affiliations
Mary E. M. Cromwell, Eric Hilario, Fred Jacobson
Keywords
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