PROTEOMIC ANALYSIS OF HUMAN BLOOD AND URINE IN DIABETIC NEPHROPATHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objective: To investigate the proteomics status of human blood and urine in diabetic nephropathy.Methods: In the present study 90 patients were selected. The study was comprised of 30 Diabetic mellitus (DM) with microalbuminuria patients (Group 3), 30 DM without microalbuminuria patients (group 2), 30 healthy controls (Group 1). Fasting glucose, post prandial glucose, lipid profile, fructosamine in serum and micro albumin in urine were investigated in all the patients.Results: The significant increase in serum fructosamine, fasting and post prandial glucose levels along with increased microalbuminuria observed in group 3 patients compared to group 2 and group 1 patients. Hyperglycemia increases fructosamine, cholesterol, triglycerides with decrease in HDL-cholesterol levels, indicates the major risk of atherogenicity. To study the effect of age, smoking, DM duration on DN, patients were grouped in different way and analysed.Conclusion: The results suggested that smoking, age and prolonged DM influences DN. Normal and DN patients serum and urine samples were selected and protein was separated by SDS-PAGE and identified by LC-MS. Results of LC-MS showed the difference in proteomics of normal and DN patients.
Authors and Affiliations
Rajesh K. S. , Dheeba B. , Sampathkumar P, Sivakumar R.
Keywords
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