Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method
Journal Title: Journal of BioScience and Biotechnology - Year 2012, Vol 1, Issue 3
Abstract
A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – ammonium hydroxide (80:80:40:0.05, v/v/v/v). The results obtained by this method (CV% 3.4) corresponded well with those obtained by using a HPLC method. The reliability of the proposed method was proved through reproducibility test with alkaloid extracts from Fumaria spp.
Authors and Affiliations
Radka Vrancheva, Ivan Ivanov, Andrey Marchev, Atanas Pavlov
Keywords
Related Articles
- EP ID EP87218
- DOI -
- Views 110
- Downloads 0
Recent advances in Broomrapes research (ERRATUM)
SSR marker-based molecular characterization and genetic diversity analysis of aromatic landreces of rice (Oryza sativa L.)
Molecular characterization of the genotypes gives precise information about the extent of genetic diversity which helps in the development of an appropriate breeding program. In the present study, a total of 24 SSR marke...
Research on the daily activity of the stone marten (Martes foina Erxl.) in anthropogenically influenced habitats in Bulgaria
The study was done on a kind of a small predator by digital photo cameras in three remote geographic areas. There have been 709 photos of stone marten (Martes foina), of which 518 in Sinite Kamani Nature Park, 118 in Sta...
Cultivation of diploid and tetraploid hairy roots of Datura stramonium L. in stirred tank bioreactor for tropane alkaloids production
Biomass accumulation and tropane alkaloids production by diploid and tetraploid hairy root cultures of Datura stramonium L. cultivated in stirred tank bioreactor at different aeration rates were investigated. The maximal...
Production of itaconic acid by Ustilago maydis from agro wastes in solid state fermentation
Itaconic acid (IA) is one of the hopeful substances within the cluster of organic acids. IA is used in artificial glass, bioactive compounds in pharmacy, medicine, agriculture, for the synthesis of fiber, resin, pl...