Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles

Journal Title: International Journal of Medical Laboratory - Year 2016, Vol 3, Issue 1

Abstract

Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin (βHCG) in the clinical samples was quantified by traditional enzyme-linked immunosorbent assay (ELISA), and then checked by the nano-immunoassay. Results: The Pearson’s correlation coefficient between ELISA and nano-immunoassay was high, i.e., 0.80. Relative sensitivity and specificity of this nano-immunoassay were reported 97.4% and 96.6%, respectively. Conclusions: BSA NPs can be applied in nano-immunoassays as a new structure, and as an example, βHCG can be detected by this novel assay.

Authors and Affiliations

Ali Jebali, Bahram Kazemi, Seyedhossein Hekmatimoghaddam

Keywords

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  • EP ID EP212427
  • DOI -
  • Views 159
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How To Cite

Ali Jebali, Bahram Kazemi, Seyedhossein Hekmatimoghaddam (2016). Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles. International Journal of Medical Laboratory, 3(1), 13-19. https://europub.co.uk./articles/-A-212427