QUANTITATIVE ANALYSIS OF Α-MANGOSTIN IN MANGOSTEEN (GARCINIA MANGOSTANA L.) PERICARP EXTRACT FROM FOUR DISTRICT OF WEST JAVA BY HPLC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: The objective of this study was to determine the validity of analytical methods of α-mangostin using HPLC with UV detector and acidic methanol as mobile phase. Then, compare the level of α-mangostin and quality of mangosteen pericarp from four regions in West Java.Methods: The determination was performed by HPLC with C-18 (octadecylsilane) as a column with methanol and 1% acetic acid in water (95:5) as mobile phase and a flow rate of 1 ml/min. UV detector was adjusted at 246 nmResults: The calibration curves for the α-mangostin were linear over concentrations range from 5 to 200 µg/ml with a correlation coefficient (r) 0.9999. The coefï¬cient of variation obtained from α-mangostin was less than 2 %. LOD and LOQ of α-mangostin were 2.122 and 7.072 μg/ml, respectively. The levels of α-mangostin from four regions were 12.39 % for Subang, 8.30 % for Purwakarta, 6.34 % for Bogor and 5.70 % for Tasikmalaya.Conclusion: The modified HPLC method meets the validation criteria. The highest a-mangostin level and the best pericarp quality was mangosteen from Subang.
Authors and Affiliations
Muchtaridi Muchtaridi, Devi Suryani, Warid Ali Qosim, Nyi Mekar Saptarini
Keywords
Related Articles
- EP ID EP576137
- DOI -
- Views 55
- Downloads 0
ANTIOXIDANT STUDIES ON METHANOL AND AQUEOUS EXTRACTS OF GYMNOSPORIA MONTANA PLANT
Objective: The main objective of this study was to evaluate the free radical scavenging activity of methanol (70%) and aqueous extract of G. montana leaves which is a traditionally used herb known for its hepatoprotectiv...
EFFICACY OF UNANI FORMULATIONS IN CHRONIC ENDOCERVICITIS: A RANDOMIZED COMPARATIVE STUDY
Objective: To compare the efficacy of Unani formulation in chronic endocervicitis.Methods: A parallel, comparative randomized trial was conducted at Govt. Nizamia General Hospital, Hyderabad from 1998 to 2000 in 50 patie...
MICROWAVE ASSISTED EXTRACTION; PHYTOCHEMICAL EVALUATION OF MALAYSIAN PALM OIL TRUNK EPIPHYTES FERNS
Objective: The objective of this research was to evaluate the, total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity, cytotoxicity and functional group of epiphytes ferns grows on the Malays...
EFFECT OF VARYING CONCENTRATION OF HERBAL EXTRACT OF NYCTANTHES ARBOR-TRISTIS LEAF ON SYNTHESIS OF SILVER NANOPARTICLES AND ITS EVALUATION
Objective: Synthesis of silver nanoparticles (AgNPs) using N. arobor-tristis aqueous leaf extract with the aid of micro wave is being demonstrated here.Methods: UV-visible spectrophotometer, dynamic light scattering (DLS...
INDUSTRIALIZATION OF MEDROXY PROGESTERONE ACETATE IN PROLONGED PARENTAL SUSPENSION (PART I)
Objective: Medroxy progesterone acetate (MPA) is structurally related to progesterone it is given by mouth or by intramuscular (IM) as a contraceptive in an aqueous suspension injection. It was reported that the MPA susp...