Quantitative variations in ovarian follicles of female Sprague Dawley rats after exposure to low dose gamma radiation
Journal Title: Journal of Applied and Natural Science - Year 2014, Vol 6, Issue 1
Abstract
In the present experiment, an attempt was made to assess the genetic risk of low dose radiations in mammals. For this purpose female Sprague Dawley rats, 11-12 weeks old, were irradiated with whole body Co 60 gamma rays in three fractionated doses of 0.10 Gy (cumulative dose 0.30 Gy) given at an interval of one month at two different dose-rates (0.00368 Gy/min. and 0.0589 Gy/min.). Ovaries were studied for quantitative evaluation of follicles at 1, 4, 12, 28 and 52 weeks after last fractionated exposure. Quantitation revealed lower number of ovarian follicles in irradiated animals than in controls. The follicular number decreased with the advancement of time after last exposure (i.e. 2 months) and reached a peak level on 28 weeks. After that the recovery was evident but the number remained below 25% of total follicles even at 52 weeks autopsy interval, which indicated an irreversible damage in ovarian tissue. Primary follicles were found to be the most radiosensitive among the various types of follicles. The highest loss in these follicles was noted at 12 weeks after exposure with the high dose-rate, where only 10.61% of them were scored. Dose-rate exhibited an inverse relationship with the number of surviving follicles. At the higher dose-rate (0.0589 Gy / min.), depletion in the total follicular number was significantly higher than at the low dose-rate (0.00368 Gy/min.) used.
Authors and Affiliations
S. Singh, J. Sharma, P. Goyal
Keywords
Related Articles
- EP ID EP116092
- DOI 10.31018/jans.v6i1.375
- Views 104
- Downloads 0
Impact of changing ecophysiological conditions in blood urea levels of freshwater fish Wallago attu
Definite cyclic variations were observed in blood urea levels of freshwater catfish Wallago attu. The maximal mean blood urea level (8.44±1.96 mg/100 ml) was noted in the month of June while minimal level in December, re...
Role of sustainomics and external climate forcing mechanism in biodiversity extinction : An overview
Over millions of years, physiology and anatomy of the living organisms has been changed due to internal climate forcing mechanism. This has influenced the world wide distribution of species. External climate forcing mech...
Optimization of fermentation conditions for producing Indian rock bee (Apis dorsata) mead using response surface methodology
Mead is a traditional drink which results from the alcoholic fermentation of diluted honey carried out by yeast (Saccharomyces cerevisiae KF233529). The present investigation was carried out for the optimization of ferme...
Assessment of nutrient ratios in the leaf of sugarcane grown in Theni district of Tamil Nadu, India
To assess the impact of nutrient ratios N/P, N/K and N/S on sugarcane productivity, a survey was conducted during its grand growth stage (October, November and December) influenced by various yield levels based on soil q...
Response of wheat cultivars to foliar potassium fertilization under irrigated saline environment
A field experiment with split-split plot design (SSPD) was conducted to study the response of two winter wheat (Triticumaestivum L.) cultivars (viz. salt tolerant cultivar KRL-1-4 and salt non-tolerant cultivar HD-2894)...