Quercetin Decreases Th17 Production by Down-Regulation of MAPK- TLR4 Signaling Pathway on T Cells in Dental Pulpitis
Journal Title: Journal of Dentistry, Shiraz University of Medical Sciences - Year 2019, Vol 19, Issue 4
Abstract
Statement of the Problem: Quercetin is a pharmacological flavonoid that can inhibit high mobility group box1 (HMGB1) protein, a non-histone nuclear protein that is implicated in inflammation. Th17 cells are important cells in the pathogenesis of inflammation. Pulpitis is the inflammation of dental pulp, which usually is accompanied by pain. Quercetin may alleviate this inflammation. Purpose: The current study aimed to compare blocking of HMGB1 function and stimulation of HMGB1 function with quercetin and investigate the effects of the blockage on T helper 17 (Th17) cells and mitogen-activated protein kinase Toll-like receptor 4 (MAPK-TLR4) signaling pathway. Materials and Method: T cells isolated from the pulp involved with pulpitis and the normal pulp were cultured. The cells suspensions were plated in 6-wells culture plates and stimulated with 0.5 µg/ml of HMGB1 for 2, 4, 8, and 12 hours. For blocking TLR4, 10 µg/ml rabbit anti-human TLR4 antibody was added 1 hour before treatment with HMGB1. Results: The level of these cytokines decreased; moreover, western blot data showed that quercetin could decrease MAPK signaling pathway by means of inhibition of HMGB1 on T cells. The results showed the reduction of TLR4 pathway and Th17 cell polarization. Conclusion: Our results indicated that the levels of IL-17, IL-33, and IL-6 in supernatants from patients’ cultured T cells were increased after stimulation with HMGB-1 following employing quercetin. It also could inhibit MAPK signaling pathway, which subsequently could decrease Th17 production and IL-17. Quercetin could decrease pro-inflammatory cytokines and IL-17 production.
Authors and Affiliations
Ali Mahmoud Hashemi, Shiva Solahaye Kahnamouii, Hassan Aghajani, Khalil Frozannia, Alireza Pournasrollah, Rasoul Sadegh, Heidarali Esmaeeli, Yosef Ghadimi, Ebrahim Razmpa
Keywords
Related Articles
- EP ID EP419901
- DOI -
- Views 70
- Downloads 0
Comparison of Endodontic Medicaments on Bond Strength of Fiber Post to Root Dentin Using Resin Cement
Statement of the Problem: Endodontic irrigants and medicaments may affect the bond strength of intracanal posts to root dentin. Purpose: The aim of this study was to compare the effect of calcium hydroxide (Ca(OH)2) and...
Solitary Fibrous Tumor of Retromolar Pad; a Rare Challenging Case
Solitary fibrous tumor has a wide spectrum of histopathologic features and many tumors show similar microscopic features. This similarity poses diagnostic challenges to the pathologists and immunohistochemical analysis i...
Granular Cell Odontogenic Tumor, an Extremely Rare Case Report
The granular cell odontogenic tumor is an extremely rare odontogenic neoplasm which about 38 cases has been reported with sufficient documentation in the literature. It has a prominent predilection to occur in the poster...
Comparison between Glenoid Fossa Roof Thickness in TMD and non-TMD Patients, a CBCT Study
Statement of the Problem: Various pathologic conditions can affect the thickness of the roof of glenoid fossa (RGF). The relationship between the RGF thickness and temporomandibular joint disorder (TMD) is not explicitly...
Activation Toll-Like Receptor7 (TLR7) Responsiveness Associated with Mitogen- Activated Protein Kinase (MAPK) Activation in HIOEC Cell Line of Oral Squamous Cell Carcinoma
Statement of the Problem: Oral squamous cell carcinoma is the most common oral malignancy. Toll-like receptor (TLR) activation led to alterations in the levels of mRNA encoding the TLR accountable for recognizing the ind...