Rapid Detection of MRSA by Loop Mediated Isothermal Amplification in Bovine Milk Samples
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 1
Abstract
Aim: To develop a simple and rapid detection method for diagnosis of MRSA in bovine milk samples suspected for mastitis. Methodology: The laboratory sensitivity and specificity of LAMP assay was carried out using available laboratory strains. Milk samples were collected from Thiruvallur and Kanchipuram districts of Tamilnadu, India. DNA was directly isolated from the milk samples and mecA gene was screened by both PCR and LAMP methods. Results: The LAMP assay successfully amplified the mecA gene under isothermal conditions at 64°C within 60 min. LAMP assay was able to detect 10 pg of DNA and did not amplify mecA gene from bacterial DNA of other species. The screening of milk samples for MRSA showed 47 Positive out of 77 Samples by PCR and 43 positive out of 77 Samples by LAMP. Conclusion: Application of LAMP assay enabled rapid and easy detection of MRSA in milk samples suspected for bovine mastitis.
Authors and Affiliations
G. Sathish, E. Hemakumar, Kurunchi C. Divya
Keywords
Related Articles
- EP ID EP350913
- DOI 10.9734/BMRJ/2016/28037
- Views 98
- Downloads 0
Effects of Water Treatment Methods on Protein Profile of Escherichia coli O157:H7 and Escherichia coli Non-O157 Isolated from Drinking Water Sources in Ado-Ekiti, Ekiti State, Nigeria
Aim: To examine the effects of water treatment methods on the protein profile of Escherichia coli O157 and Escherichia coli non-O157 isolated from different drinking water sources in Ado-Ekiti. Study Design: Experimenta...
Comparison of two DNA Extraction Protocols Representing Bacterial Community from Bulk Soil by PCR-DGGE
Aims: The objective of this work was to investigate the efficiency of DNA extraction from bulk soil using the Britânia® mini mixer combined with phenol extraction to study bacterial communities, from three sampling sites...
Bacterial and Fungal Contaminants in Mycobacterium tuberculosis Cultures at National Tuberculosis Referral Laboratory-Kampala
Aim: This study was aimed at identification of fungal and bacterial contaminants in Tuberculosis cultures at the Uganda National Reference Laboratory (NTRL). Methods: The cultures were previously grown on Lowenstein-Jen...
Prevalence of Methicillin Resistant Staphylococcus aureus among Isolates from Wounds in Surgical Wards at Kabale Regional Referral Hospital, South Western Uganda
Background: Methicillin resistant S. aureus (MRSA) is a multidrug resistant organism that threatens the continued effectiveness of antibiotics worldwide and causes a threat almost exclusively in hospitals and long-term c...
Bacteremia in Children Infected with HIV/AIDS in Minna, Niger State, Nigeria
Bacterial infection was reported to be one of the leading causes of high mortality among children. Bacteremia is a frequent complication found among HIV infected children and usually associated with a poor prognosis. Thi...