Reactivity of commercially available monoclonal antibodies to human CD antigens with peripheral blood leucocytes of dromedary camels (Camelus dromedarius)
Journal Title: Open Veterinary Journal - Year 2017, Vol 7, Issue 2
Abstract
Monoclonal antibodies (mAbs) to cell surface molecules have been proven as a key tool for phenotypic and functional characterization of the cellular immune response. One of the major difficulties in studying camel cellular immunity consists in the lack of mAbs that dtect their leukocyte differentiation antigens. In the present study two-parameter flow cytometry was used to screen existing commercially available mAbs to human leukocyte antigens and major histocompatibility molecules (MHC) for their reactivity with camel leukocytes. The comparison of patterns of reactivity obtained after labelling human and camel leukocytes have shown that mAbs specific to human cluster of differentiation (CD) 18, CD11a, CD11b and CD14 are predicted to be cross-reactive with homologous camel antigens.
Authors and Affiliations
Jamal Hussen| Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Al Ahsaa, Saudi Arabia, Turke Shawaf| Department of Clinical Studies, College of Veterinary Medicine, King Faisal University, Al Ahsaa, Saudi Arabia, Abdulkareem Imran Al-herz| Immunology Unit, Diagnostic Laboratory and Blood Bank, King Fahad Hospital Hufof, Al Ahsaa, Saudi Arabia, Hussain R. Alturaifi| Immunology Unit, Diagnostic Laboratory and Blood Bank, King Fahad Hospital Hufof, Al Ahsaa, Saudi Arabia, Ahmed M. Alluwaimi| Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Al Ahsaa, Saudi Arabia
Keywords
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- EP ID EP9421
- DOI http://dx.doi.org/10.4314/ovj.v7i2.12
- Views 386
- Downloads 24
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