Reassessment of Clinical Pathogens Diagnosed by Sub-urban Facilities of Dhaka: Necessity of Comprehensive Techniques to Manage Antibiotic Resistance
Journal Title: Microbial Bioactives - Year 2018, Vol 1, Issue 2
Abstract
Background: The determinative bacteriology currently available in the suburbs of Dhaka city mainly involves culture-based identification techniques. Incorporation of extensive biochemical characterization could enhance the efficiency of the existing methods and reduce the risk of wrong medication. The study was aimed, in this connection, at reassessment of clinical pathogens in the suburbs of Dhaka city through biochemical and molecular analysis. Methods: To assess the accuracy of identification of clinical pathogens by the diagnostic facilities of suburbs of Dhaka city, we were provided with previously identified clinical strains from different diagnostic facilities along with their clinical data. The etiological agents, were analyzed based on the cultural characteristics on different selective agar media and biochemical properties. The API20E profiles of the pathogens were analyzed to identify the organisms. Furthermore, to verify the results of API20E, 16s rRNA genes were sequenced and their phylogenetic relationship was checked in NCBI database. Result: The gram-negative clinical strains that the diagnostic facilities (DFI) provided were Escherichia coli, Klebsiella, and Pseudomonas. We further reassessed their identities among which two-third of those clinical strains were correctly identified as E. coli while half of those were correctly reported as Klebsiella. In addition to it, some of the DFI strains were also identified as Enterobacter, Yersinia, Acinetobacter, and as well as some unknown bacterial genera. These results were confirmed initially by biochemical tests followed by API20E and 16s rRNA sequencing. Finally, through antibiogram we also observed that the reconfirmed E. coli and Klebsiella strains were resistant to various antibiotics, such as ampicillin, cefotaxime, ciprofloxacin, azithromycin etc. Conclusions: Our findings allude to the fact that diagnostics facilities though are able to identify gram-negative bacteria within clinical strains, they are unable to identify the causative agents properly. We also hypothesize that misidentification of bacterial pathogens may promote the dissemination of antibiotic resistance.
Authors and Affiliations
Shanaz Fatema Bristy, Md. Reaz Uddin, Siraje Arif Mahmud, Md. Jalal Uddin, Ali Azam Talukder, Mohib Ullah Khondoker, Mohd. Raeed Jamiruddin, Nafisa Azmuda, Nihad Adnan
Keywords
Related Articles
- EP ID EP409511
- DOI 10.25163/microbbioacts.12013C0207101018
- Views 42
- Downloads 0
Pasteurization of Milk through Direct Heating up to 75°C over Kitchen Stove at Home
Pasteurization remains as an important process that makes milk safe from pathogens and increases milk’s shelf life without altering the taste and the nutritional value. But, still many people drink unpasteurized milk eit...
Antimicrobial efficacy of commercially available dentifrices against newly isolated Streptococcus mutans
Background: The aim of the present study was to evaluate the anticariogenic activities of commercially available dentifrices such as, toothpastes and mouthwashes against the most prominent cariogenic bacteria S. mutans....
Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules
Cancer treatment remains as an expensive process due to the cost of sophisticated infrastructure development as well as its maintenance with skilled personnel. At the same time, the success rate is not very inspiring sin...
Bioactive potential from Marine sponge Callyspongia diffusa associated Pseudomonus fluorescens BCPBMS-1 and Penicillum citrinum
The exploration for marine sponge associated novel microbes, producing rich and highly potential therapeutic metabolites, could diversify the scopes in life sciences. Since this has remained mostly untouched, the researc...
Physico-chemical Characterization of Indigenous Streptomyces and Influence of pH on Antimicrobial Activity
Background: Emergence of multi-drug resistant pathogens has afflicted the population of developing countries like Bangladesh in recent years for which a sustainable holistic combating approach is required. Since Streptom...