REMOVE OF HEXADEC YLPYRIDINIUM BROMIDE FROM AQU EOUS SOLUT IONS BY BA CTERIA OF TH E GENUS PSEUDOMONAS IN THEIR INTERACTION WITH CLAY MINERAL AND CHITOSANH CLAY MINERAL AND CHITOSAN
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 1
Abstract
The aim. Improvement of the method of removing the most common representative of cationic surfactants – biologically hard hexadecylpyridinium bromide (HDPBr) from aqueous solutions of oil oxidative bacteria of the genus Pseudomonas in their interaction with the natural sorbents (clay mineral and chitosan). Methods. Nonpathogenic bacteria P. fluorescens ONU328, P. maltophilia ONU329, P. cepacia ONU327 were cultivated in nutrient medium M-9 with the addition of 0.5% peptone at pH of 7.2 to 7.4. After sorbtion the residual content of cationic surfactants in solutions was determined by the colorimetric method. Results. In result of studies it is established that the use of bacteria P. cepacia ONU327, P. fluorescens ONU328 and P. maltophilia ONU329 in their interaction with the natural sorbents (clay mineral and chitosan 1:1 by weight) allows to increase in neutral environment a degree of removal HDPBr from aqueous solutions to 98.0–99.5% at the initial concentration of cationic surfactant 100 mg/l and to reduce the optimal time contact with the sorbents from 60 min in the absence of microorganisms to 20 minutes at their presence. With help of 1 g of mixed sorbent it is removed 24.5–24.9 mg of cationic surfactant from the solution. Conclusion. Using of biologically modified natural sorbents provides deep water purification from cationic surfactant, from salts аlkylpyridinium in particular, and the stage of sorbtion is recommended to includ in a comprehensive, ecologically safe biotechnology of water purification.
Authors and Affiliations
T. V. Gudzenko, O. V. Voliuvach, T. A. Beliaeva, O. G. Gorshkova, I. V. Puzyreva, V. О. Іvanytsia
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