Resazurin assay for rapid drug susceptibility testing of Mycobacterium tuberculosis
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 1
Abstract
Introduction Tuberculosis a disease of antiquity continues to cause high morbidity and mortality In Sustainable Development Goals and End Tb Strategy proposed by WHO includes Drug Susceptibility Testing as major component Conventional methods are time consuming and molecular methods are rapid but expensive and demanding Resazurin assay is a rapid colorimetric redox indicator method of Drug Susceptibility TestingMaterials and Methods 67 strains of M tuberculosis isolated from pulmonary tuberculosis patients were tested using indirect absolute concentration method by resazurin assay and compared with drug incorporated Lowenstein Jensen media for Isoniazid and RifamycinResults There was 915 and 917 concordance between both methods for INH and Rifamycin respectively Resazurin assay for INH was 100 sensitive and 6154 specific Sensitivity and specificity for RMP was 9804 and 625 respectively The duration required for INH was 70 plusmn 207 and for RMP 658 plusmn 158 daysConclusions Resazurin assay is sensitive rapid cheap technically simple drug susceptibility methodKeywords M tuberculosis resazurin assay drug susceptibility
Authors and Affiliations
Mangala Ghatole, Virendra Kashetty, Apeksh Ghule
Keywords
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- EP ID EP473853
- DOI 10.18231/2394-5478.2018.0028
- Views 87
- Downloads 0
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