Resistance of Escherichia coli isolates from the surface of the pork carcasses to the antibacterial preparations
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. Taking into account that in Ukraine there is insignificant amount of research in this direction, we decided to carry out a study on the distribution E. coli (AMR), isolated from pork production resistance to antibiotics. The goal of the work was to study resistance of E. coli isolates to antibiotics that were isolated from the surface of pork carcasses at meat processing factories (slaughterhouses) of Odessa and Sumy regions. Materials and methods. Sampling was carried out at slaughterhouses of Odessa and Sumy oblasts in 2017 . Microbiological studies were carried out at the Odessa regional laboratory of veterinary medicine and in the accredited microbiological laboratory of the Department of Public Health of Sumy State University. Results of research and discussion. The 645 samples from surfaces of pig carcasses were investigated: 318 from the Odessa region and 327 from the Sumy region. The samples were taken twice: before the chilling of pig carcasses and at least in 12 hours after slaughtering, making swabs from the area of 100 cm2. 645 samples were taken randomly from 3–5 carcasses according to the rules of asepsis and ISO 17604, using a physiological solution. The samples were investigated in the laboratory in the first 2 hours after sampling. To isolate E.coli were carried out using the “Compact Dry” Petri dishes with a selective agar for coliforms and E.coli (NISSUI pharma). Disk diffusion method was used to determinate resistance of E.coli isolates to 10 antibiotics (penicillin (10 mcg), methicillin (15 mcg), vancomycin (30 mcg), lincomycin (15 mcg), oxacalin (20 mcg), gentamicin (10 mcg), onloxacin (10 mcg), rifampicin (15 μg), ampicillin (10 μg), streptomycin (10 μg)). In addition to the disk diffusion method, antibiotic susceptibility testing of isolates was performed by the MIS E-test-HiComb for E.coli (TM Himedia). In this case, the values of MIC (minimal inhibitory concentration) for isolates of E.coli were determined. The E. coli were isolated in 15,3% (49) samples and 19,2% (63) samples respectively. E.coli was detected in 15,3% of samples from Odessa oblast and 19,2% ones from Sumy oblast. In the swabs from the surfaces of pig carcasses, isolated from the Odessa oblast were isolated AMR E.coli in average 34.7% of cases, and samples from Sumy oblast in 53.7%, the greatest resistance of isolates were shown to ampicillin – 67.3% in Odessa oblast, and 80.6% in Sumy oblast. A greater number of resistance in E.coli isolates was found in relation to penicillin, methicillin, ampicillin from 46.9% to 67.3% of cases in the Odessa oblast, and to penicillin, methicillin, ampicillin, oxazalin and streptomycin 71.4–80.6% of cases in Sumy oblast. Isolates of AMP E.coli ranged from 45% to 47% of cases showed resistance to antibiotics in 32% of cases, they were resistant to 2 antibiotics and showed the lowest level resistance to 4 antibiotics. Isolates of AMP E.coli, which were isolated from carcasses in Odessa and Sumy oblasts, had no resistance to ofloxacin in 95.9% and 95.2% of cases. Conclusion and prospects for further research. The prospect of further work is to carry out research on the distribution of E. coli AMP in the pork meat production chain and to substantiate the measures to control spreading of these microorganisms.
Authors and Affiliations
Victoria Kasіanchuk, Alexandra Berhilevich, Volodymyr Kusturov, O. Deryabin
Keywords
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- EP ID EP404722
- DOI 10.31073/vet_biotech32(2)-26
- Views 124
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