Resistance prevalence profile of Klebsiella pneumoniae in the Intensive Care Units of AlShatby Pediatric Hospital, Alexandria, Egypt
Journal Title: Novel Research in Microbiology Journal - Year 2019, Vol 3, Issue 6
Abstract
Klebsiella pneumoniae has been identified as an opportunistic pathogen associated with both communityacquired and nosocomial infections mainly among patients admitted to the Intensive care units (ICUs). Some resistant genes were transferred vertically or horizontally within many microbial communities, directly between bacteria through plasmids or integrin. Different techniques including; phenotypic and genetic ones were used to evaluate the presence of β-lactamases among the isolated strains of K. pneumoniae. This bacterial species is the most commonly isolated pathogen (95 isolates) from all the examined samples (51.35%). Current results revealed that 30 and 14 strains of K. pneumoniae are positive to Extended spectrum β-lactamase (ESBL) production, and AmpC β-lactamase producers, respectively. On the other hand, modified carbapenem inactivation and modified Hodge test (MHT) were used to assess the carbapenem resistant strains. It is observed that all the β-lactamase producers’ strains are also carbapenemase producers, whereas only nine strains (30%) are MHT positive. The Polymerase Chain Reaction (PCR) technique revealed that TEM, BETA, NDM and IPM genes are found on the bacterial plasmid (100%). However the presence of the β-lactamase genes on the bacterial DNA varied among the different strains. The presence of the resistance genes on the bacterial plasmid may signify the resistance acquired upon the previous exposure of this bacterium to the different antibiotics. The aims of the current work were to isolate K. pneumonia from Al-Shatby hospital ICUs, to determine the incidence of its β-lactamases, and to decide the frequency of acquisition of 12 different genes among ESBL K. pneumoniae isolates.
Authors and Affiliations
Bassma H. Elwakil; Safaa M. Ali; Soad F. Hafez; Adnan A. Bekhit; Moustafa Y. El-Naggar; Zakia A. Olama
Keywords
Related Articles
- EP ID EP691311
- DOI 10.21608/nrmj.2019.66746
- Views 92
- Downloads 0
Detection of blaNDM-1 gene among the carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates from a children’s hospital in Nepal
This study was undertaken to detect the presence of blaNDM-1 gene among the carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates, confirmed through using Modified Hodge Test (MHT). During...
Molecular, serological and biological characterizations of Potato Leaf Roll Virus in infected potato plants in Egypt, and its effects on plant cell organelles
Potato Leaf Roll Virus (PLRV) is one of the most serious viruses infecting potato plant (Solanum tuberosum L.) in Egypt. Indirect Enzyme Linked Immunosorbent Assay (Indirect- ELISA) results revealed that 70 % of the coll...
Green synthesis, Optimization and Characterization of SiO2 nanoparticles using Aspergillus tubingensis F20 isolated from drinking water
This study aimed to demonstrates a positive correlation between silica metal tolerance ability of a drinking water fungi and its potential for the synthesis of silica oxide (SiO2) nanoparticles (NPs). Metal oxide NPs ca...
Recruitment of bacterial endophytes by host plants
Plants coupled with endophytic bacteria hold great potential for the remediation of polluted environments. The colonization patterns and activities of inoculated endophytes in the rhizosphere an...
Pathogenicity and inoculum concentration effects of Clavibacter michiganensis subsp. michiganensis on severity of bacterial canker of tomato
A total of 100 tomato plant samples exhibiting typical symptoms of bacterial canker were collected from tomato-growing areas of north-western areas of Pakistan. On isolation of the causal agent of this di...