Response of tomato wild species, landraces and commercial cultivars to Meloidogynejavanicainfection as revealed by molecular and conventional approaches
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 6
Abstract
In present study, response of some commercial cultivars, landraces andwild species of tomato were evaluated against Meloidogynejavanica.Furthermore, presence of Mi-1 alleles in the plant materials was traced using PMiF3/PMiR3, Mi23F/Mi23R and REXF1/REXR2 primer pairs. In greenhouse experiments, seven genotypes (ES1002, Super Beita, Ant 93-04, Samrudhi, Nun 6108, Pascal and Solanumperuvianum[LA0111])were less affectedby the nematode while the rest figured more susceptible. Using PMiF3/PMiR3 primer pair, two fragments of 350 and 550bp were amplified in the seven genotypes, whereas in the remaining genotypes only the allele of 350 bp was generated. Based on Mi23F/Mi23R primer pair, the seven genotypes were found heterozygote for 380bp and 430bp fragments and the others showed only a single allele of 430 bp. In all the genotypes except Super Strain B when were treated with REXF1/REXR2 primer pair, a 750bp fragment was realized. Digestion of the amplified fragments with TaqI restriction enzyme resulted in the appearance of 550bp and 150bp fragments in nine cultivars, the fore mentioned seven genotypes plus a landrace (Laleh) and Solanumhabrochaites (LA1223). It can be concluded that the Mi23 and PMi3 markers are more reliable for separating the resistant genotypes from susceptible ones.
Authors and Affiliations
Shalaleh Moslehi, Gholamreza Niknam, SeyedAbolghasemMohammadi, Maghsoud Pazhouhandeh
Keywords
Related Articles
- EP ID EP38680
- DOI -
- Views 177
- Downloads 0
Essential Oil Variation within and between Stachys inflata Benth. and Stachys lavandulifolia Vahl. Populations from Iran
Although several species of Stachys possess characteristic essential oils, only a few published studies have described within and between populations of their volatile constituents. The aim of the present study was to ev...
The study of structural characteristics of Zelkova Carpinifolia (Pall.) trees in the Educational – Research forest of Darabkola– Mazandaran
In this study, the structural properties of Zelkova Carpinifolia trees were compared in pure and mixed stands of educational -Research forest of Darabkola. The inventory was performed selectivelyin 16 circular plots (10...
umerical taxonomy of selected species of Pottiaceae family in Iran
To determining the phenetic similarities between 16 species of Pottiaceae family, 20 characters have been studied by numerical taxonomy. A cluster analysis based on Average Linkage method determined two main groups . P...
Coral disease prevalence in Samui Island and the adjacent islands, southern part of the Gulf of Thailand
Preliminary surveys for coral disease were conducted in April 2012 at shallow water reefs of Samui Island (Bang Po Bay and Thong Krud Bay) and four adjacent islands (Katen Island, Jatamoon Island, Mud Sum Island and Hin...
Evaluation of species diversity in Iranian-Turanian forest islands and its relation with climate factors and natural sites bionomics (A case study of markazi province)
In order to study the species diversity and genetic diversity of Amygdalus scoparia and Pistacia atlantica, 21 thousand hectares of forest ecosystem were made in Markazi province and the effects of climatic and bionomic...