REVERSE PHASE-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF AZILSARTAN MEDOXOMIL AND CHLORTALIDONE IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: Journal of Atoms and Molecules - Year 2012, Vol 2, Issue 1
Abstract
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Azilsartan Medoxomil and Chlortalidone in pharmaceutical dosage forms. Isocratic elution at a flow rate of 0.9ml min-1 was employed on a symmetry C18 column at ambient temperature. The mobile phase consisted of Methonal: Water: Acetonitrile : 0.1% Ortho phosphoric acid 30:35:15:5(v/v/v/v). The UV detection wavelength was at 251 nm. The retention time for Chlortalidone was 3.923min and Azilsartan Medoxomil was 7.208 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Azilsartan Medoxomil and Chlortalidone in pharmaceutical dosage forms.
Authors and Affiliations
Madhu Babu Kasimala, Bikshal Babu Kasimala
Keywords
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- EP ID EP367430
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