Reversed Phase High Performance Liquid Chromatographic Method for Simultaneous Estimation of Pregabalin and Aceclofenac in Tablet Formulation (Acenac-N)

Journal Title: Journal of Reports in Pharmaceutical Sciences - Year 2014, Vol 3, Issue 2

Abstract

Pregabalin (PRG) is a new antiepileptic drug and Aceclofenac (ACE) is a potent non-steroidal anti-inflammatory drug. These drugs in combination are used for treatment of partial seizures and neuropathic pains. A simple and precise assay method by RP-HPLC was developed and validated for estimation of PRG and ACE in Acenac-N tablet. Analyses of commercial tablet, Acenac-N were performed using JASCO Isocratic HPLC system at 248 nm on a reverse phase column HiQ sil C18HS (4.6 ?— 250 mm, 5I¼m), a mobile phase; methanol: phosphate buffer (70:30 v/v, PH 3). The validation aspects were selectivity, linearity, precision, accuracy and quantification limit. Linearity, 5-25 I¼g/mL for PRG and ACE respectively, provided determination coefficients (R2) of 0.998 and 0.994 respectively, for PRG and ACE and proved precise since the RSD% was less than 2%. The recoveries obtained ranged from 99.10% to 100.90% for both of the drugs with RSD% less than 2%. The LOD for PRG and ACE was found to be 0.270 I¼g/mL and 0.039 I¼g/mL respectively. The LOQ was found at 0.818 I¼g/mL and 0.120 I¼g/mL respectively for PRG and ACE. In this study, the optimization of mobile phase, flow rate, injection volume and wavelength were achieved. The retention time for PRG and ACE was 3.241 and 6.581 min respectively. Drug content of the Acenac-N tablet was found to be 99.53% and 100.12% respectively for PRG and ACE. The method was validated as per the ICH guidelines. This method is precise, accurate and easy to analysis PRG and ACE in tablets.

Authors and Affiliations

Keywords

Related Articles

Synthesis, Molecular Docking and Cytotoxicity Evaluation of 2-(4-Substituted-Benzyl) Isoindoline-1, 3-Dione Deriva-Tives as Anticancer Agents v

The development and discovery of new anticancer agents is one of the main goals in medicinal chemistry. The conventional anticancer drugs are concomitant with high incidence of unpleasant side effects like severe gastroi...

Effect of Scopolamine and Mecamylamine on Antidepressant Effect of Rivastigmine in a Behavioral Despair Test in Mice

Rivastigmine can decrease the severity of depression in Alzheimer’s disease patients. Apart from the monoamines hypothesis of despair, it is well known that the cholinergic system is also responsible. Thus regarding the...

Electroreduction of tacrolimus as immunosuppressant agent at hanging mercury drop electrode: application to determination in pharmaceutical formulation

The adsorptive behavior of the immunosuppressive agent tacrolimus was studied by cyclic and differential-pulse voltammetry on a hanging mercury drop electrode (HMDE). The drug was accumulated at HMDE and two well-defined...

Evaluation of the Intestinal Absorption of Maltol-Zn and Ethyl Maltol-Zn Complexes in Rat

Zinc deficiency in man is characterized by skin lesion, alopecia and has been linked to growth retardation and mental disturbance. For supplementation purposes zinc is currently presented as its sulfate salt, from which...

Effect of Streptokinase on Short-term Outcomes in the Acute Myocardial Infarction with ST-Segment Elevation

Acute coronary syndrome is one of the most prevalent cardiovascular diseases. The acute myocardial infarction is one of its kinds which embodies the indication of fibrinolysis. One of the fibrinolytic types mostly used i...

Download PDF file
  • EP ID EP340975
  • DOI -
  • Views 37
  • Downloads 0

How To Cite

(2014). Reversed Phase High Performance Liquid Chromatographic Method for Simultaneous Estimation of Pregabalin and Aceclofenac in Tablet Formulation (Acenac-N). Journal of Reports in Pharmaceutical Sciences, 3(2), -. https://europub.co.uk./articles/-A-340975