Role of 4-Phenylbutyric Acid in DNA and Protein Binding and its Functional Analysis
Journal Title: International Journal of Experimental Research and Review - Year 2024, Vol 45, Issue 9
Abstract
4-Phenylbutyric acid (4-PBA) is a small molecule known for its protein folding capacity to reduce endoplasmic reticulum (ER)-stress. This study aimed to explore the potential of 4-PBA by studying its interactions with DNA and protein and examining its effects on cellular toxicity and antibacterial activity. UV-VIS absorption spectroscopy demonstrated that 4-PBA effectively binds to calf thymus DNA (CT-DNA), as indicated by an evident hyperchromic shift, suggesting stable intercalating interactions. Similarly, the fluorescence quenching assay demonstrated that 4-PBA also interacts with bovine serum albumin (BSA), reducing fluorescence intensity by occupying specific binding sites on the protein. The cytotoxicity analysis using cell counting kit-8 further showed no significant reduction in cell viability of normal human lung epithelial cell line (L132). Subsequently, 4-PBA also exhibited minimal growth inhibition of Escherichia coli and Staphylococcus aureus bacterial strains, indicating limited antibacterial activity under the tested conditions. Additionally, this study provides a basis for future research towards the molecular mechanisms and therapeutic applications of 4-PBA.
Authors and Affiliations
Dikshita Deka, Alakesh Das, Ashiq Shibili P, Antara Banerjee
Keywords
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- EP ID EP752625
- DOI 10.52756/ijerr.2024.v45spl.017
- Views 7
- Downloads 0
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