ROLE OF ANTIOXIDANT AND MYELOPEROXIDASE LEVELS IN 7, 12-DIMETHYLBENZ [A] ANTHRACENE INDUCED EXPERIMENTAL RAT MODEL: EVIDENCE FOR OXIDATIVE DAMAGE IN ACTIVE ULCERATIVE COLITIS.
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 3
Abstract
Objective: Ulcerative colitis known as inflammatory bowel disease (IBD) of unknown etiology. We examined the antioxidant and myeloperoxidase status in a murine model of 7,12-dimethylbenz[a]anthracene induced colitis to elucidate the exact mechanism behind the inflammation.Methods: Male Wistar rats were exposed to ulcerative colitis using various concentration of DMBA (7,12-Dimethylbenz[A]anthracene) were periodically analysed on 4th, 8th, 12th, 24th and 32nd week from the date of induction. To determine the disease activity index changes in body weight, food consumption, the presence of gross blood in stool and consistency of feces and diarrhea were observed. Macroscopic characters were elucidated based on clinical features of the colon and rectum using scoring pattern. Tissue inflammation status was noted through myeloperoxidase (MPO) assay. The antioxidant status in tissue samples was analysed by superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total reduced glutathione (GSH).Results: Gavage intubation of DMBA induced colitis showed significant changes from 4th week and severity on 32nd week. The body weight was gradually reduced. Macroscopic scoring showed severe scoring pattern the inflammation was significantly heavier by week 4; and by the end of 32 w, inflammation in rats was double that of the controls, tissue myeloperoxidase (MPO) activity showed the steady increase of neutrophil infiltration and inflammation rate every week. A significant change was noted in tissue antioxidant status and it showed the oxidation level. Statistically, significant change was recorded from 4th week till 32nd week.Conclusion: The conventional biochemical changes in colitis induced animal model revealed the association between the oxidative stress and ulcerative colitis.
Authors and Affiliations
P. Geetha, B. Lakshman Kumar, U. Indra, B. Pavithra Sheetal
Keywords
Related Articles
- EP ID EP575774
- DOI 10.22159/ijpps.2017v9i3.16485
- Views 76
- Downloads 0
STATISTIC ESTIMATION OF BREAKING TABLETS OF ENALAPRIL 20 MG
Objective: The aim of this study was to evaluate the accuracy of breaking into half and quarter-tablets Enalapril 20 mg sold in the Albanian market, in dosages suitable for use in children.Methods: 100 whole tablets of E...
IMPROVEMENT OF BIOAVAILABILITY OF CEFUROXIME AXETIL ORAL SUSPENSION BY INCLUSION COMPLEXATION METHOD
Objective: Cefuroxime axetil, a prodrug of Cefuroxime, is a poorly water soluble drug, thus it has got only limited solubility and dissolution rate in gastric fluids. Also, the bioavailability of Cefuroxime axetil oral s...
UNRAVELING THE POTENTIAL PHYTOCHEMICAL COMPOUNDS OF GYMNEMA SYLVESTRE THROUGH GC-MS STUDY
Objective: To profile the chemical composition of ethanolic extract of Gymnema sylvestre through Gas Chromatography-Mass Spectrometry technique.Methods: The chemical compositions of the plant leaf extracts of G. sylvestr...
PHARMACOGNOSTIC STUDY OF CALLISTEMON CITRINUS L. BARK
Objective: Pharmacognostic study of Callistemon citrinus L. barkMethods: The bark of Callistemon citrinus L. was shade dried and powdered mechanically. The powdered material was used for further Pharmacognostic study. Th...
MITIGATING ROLE OF ZINC AND IRON AGAINST CADMIUM INDUCED TOXICITY IN LIVER AND KIDNEY OF MALE ALBINO RAT: A STUDY WITH REFERENCE TO METALLOTHIONEIN QUANTIFICATION
Objective: The present study is carried out to know the mitigating role of zinc (Zn) and / or iron (Fe) supplementation on cadmium (Cd) induced toxicity in rats with special reference to metallothionein (MT) protein.Meth...