ROLE OF DEFENCE RELATED ENZYMES AND LIGNIN IN HOST RESISTANCE OF DURUM WHEAT CULTIVARS INFECTED BY Bipolaris sorokiniana
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 8
Abstract
Spot blotch, caused by Bipolaris sorokiniana, is a major disease of wheat in humid and warmer regions of the world. Host resistance is the most important trait in the host pathogen interaction. The aim of this research was to elucidate the potential biochemical parameters linked to Bipolaris resistance in the resistant NIDW295, PDW314 and susceptible Bijaga yellow, A-9-301 durum wheat cultivars. Analysis of defence enzymes like the phytochemical precursor enzyme, phenylalanine ammonia lyase (PAL), pathogenesis related proteins like, peroxidase (POX), along with the content of lignin, phenols and total chlorophyll presented significant differences between healthy and inoculated leaves (48 hrs post inoculation). Significant variations in PAL, POX activities, phenolic and lignin content were found between resistant and susceptible cultivars. Strong induction of PAL and POX activities were recorded in resistant cultivars NIDW295 (356.7%, 184.3%), PDW314 (253.6%, 156.6%) whereas minimal response was observed in the susceptible cultivars. The cell walls of NIDW295, PDW314 contained elevated levels of lignin and was observed to be more than 20 times higher with respect to Bijaga Yellow and A-9-301. The total phenolic content was highest for NIDW295, followed by PDW314, Bijaga Yellow and A-9-301. The reduction of total chlorophyll was significantly higher in susceptible genotypes in comparison to resistant genotypes. Our results suggest that these biochemical parameters (induction of PAL, POX enzyme activities, accumulation of lignin and phenols) are related to resistance to Bipolaris sorokiniana infection and indicate the perspective of use of these traits for the selection of resistant wheat genotypes.
Authors and Affiliations
K. K. MIRAJKAR
Keywords
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