Role of Sildenafil in Acceleration of Delayed Union Fracture Healing on Sprague-Dawley Rats Model
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 8, Issue 5
Abstract
Introduction: Delayed union with its subsequent morbidity remains a major problem in fracture healing. Angiogenesis plays an important role in fracture healing. Sildenafil has been shown to be a potent stimulator of angiogenesis through upregulation of pro-angiogenic factors known as vascular endothelial growth factor (VEGF). This study evaluated the role of sildenafil in accelerating the healing process in delayed union model. Methods: This was an experimental study in delayed union femoral fracture model in male Sprague Dawley rats evaluated by histomorphometry and immunohistochemistry. Twenty four rats were randomized into four groups; control (group 1), administration of sildenafil 3.5 mg/kgbw (group 2), sildenafil 5 mg/kgbw (group 3), and sildenafil 7.5 mg/kgbw (group 4). The parameters evaluated were total area of callus, osseous, cartilage, fibrous tissue, and VEGF expression. The evaluation was carried out at week-2 and -4 after intervention. Results: On week-2 evaluation, ANOVA test showed a significant difference in the total callus area with the p-value of 0.004. ANOVA test also found significant difference among groups in the osseous, cartilage, and fibrous tissue area with p-value of 0.001, 0.015 and 0.005 respectively. On week-4, ANOVA test found no significant difference among all groups in the total callus area with p-value of 0.192. However, in ANOVA test, we found significant difference between groups in the osseous and fibrous tissue area with p-value of 0.015 and 0.001 respectively. Conclusion: Sildenafil is proven to accelerate fracture healing of delayed union and to increase VEGF expression.
Authors and Affiliations
Ahmad Fauzi, Achmad Fauzi Kamal, Aryadi Kurniawan, Evelina Kodrat
Keywords
Related Articles
- EP ID EP348047
- DOI 10.9734/BJMMR/2015/15427
- Views 59
- Downloads 0
Hyperhomocysteinemia in Chronic Kidney Disease Patients in a Teaching Hospital in Nigeria
Aim: To determine the prevalence of hyperhomocysteinemia amongst chronic kidney disease (CKD) patients at a Teaching Hospital in Nigeria and to determine its relationship with the severity of kidney disease. Study Design...
The Knowledge, Attitude and Perception towards Epilepsy amongst Health Workers in a Tertiary Hospital in Uyo, Southern Nigeria
Background: Epilepsy is usually stigmatized in the developing world because of the poor knowledge. The health workers in Nigeria are generally regarded as the custodians of medical information irrespective of their desig...
Immunophenotyping of Human Immunodeficiency Virus-infected Patients with Mycobacterium Tuberculosis Co-infection
Human immunodeficiency virus (HIV) and Mycobacterium tuberculosis (MTb) infections are two human dilapidatory illnesses globally, but more worrisome is how to diagnose them and monitor disease progression in patients eff...
New Vision for the Dignity: Understanding the Meaning of Patient Dignity in Iran
Aim: Respect for human dignity right has been accepted as a fundamental human need in the world, but its meaning is complex. The present research aimed to understand the meaning of patient dignity in Iran. Study Design:...
Correlation of Anthropometric Indices and Age with Fasting Plasma Glucose among Inhabitants of Ogun State, South-West Nigeria
Background: Type 2 diabetes has assumed epidemic proportion. Several reports have linked both general and central obesity with diabetes mellitus but there are ethnic differences between adiposity, visceral adipose tissue...