Roleof Cartridge-Based Nucleic Acid Amplification Test (Cbnaat) For Early Diagnosis of Tuberculosis: A Retrospective Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 11
Abstract
Background: About one fifth of global Tuberculosis (TB) burden present in INDIA and it is ninth leading cause of death worldwide. It is very important to early diagnose and treat Tuberculosis cases to stop transmission. Smear microscopy is the cornerstone but sensitivity and its inability to detect drug resistance limits its role in TB controland it has only modest sensitivity and poor positive predictive value. Culture is the gold standard but its results typically not available for 2-6 weeks. We compared the cartridge-based nucleic acid amplification test (CBNAAT) results for diagnosis of pulmonary and extra pulmonary tuberculosis with the conventional methods like sputum smear and culture examination. Material and Methods: We conducted a retrospective study in department of pulmonary medicine, BURDWAN MEDICAL COLLEGE BURDWAN, WEST BENGAL, and RAJENDRA INSTITUTE OF MEDICAL SCIENCES, RANCHI, JHARKHAND, INDIA to analyse the result of CBNAAT in diagnosis of tuberculosis from Jan2017- Dec 2017. Data was collected from DOTS centre and CBNAAT centre. A total no of 200 cases were studied. Results: Total 200 samples for CBNAAT taken for this study. Mean age of the study population was 30.3±9.24 years. Of these 200 samples, 173 were sputum/BAL samples and 27 were extra pulmonary samples. We found rifampicin resistance rate of 4.6% (8/173) in pulmonary tuberculosis cases, no rifampicin resistance detected in extra pulmonary samples. CBNAAT could identify 43 cases (24.8%) that were smear negative. We found TBHIV coinfection rate of 3.33%. 38(21.96%) Sputum/BAL samples were AFB smear positive and 135(78.03%) % were negative. Chi square test was applied; P value is <0.001. For pulmonary samples, the sensitivity and specificity for CBNAAT samples were 80.2% and 90.6% respectively; while that for sputum smear were 42.6% and 98.1% respectively. For extrapulmonary samples, the sensitivity and specificity for CBNAAT samples were 84.9% and 94.2% respectively; while that for sputum smear were 61.3% and 100% respectively. Conclusions: We found CBNAAT is an important diagnostic modality especially in sputum negative patients for early diagnosis and treatment in lesser time as compared to conventional sputum microscopy. It also detects the rifampicin resistance with high specificity to start early treatment.
Authors and Affiliations
Manish Kumar Munda, Jitendra Singh, pramod Kumar, Shashi Kant Suman
Keywords
Related Articles
- EP ID EP406777
- DOI 10.9790/0853-1711015052.
- Views 74
- Downloads 0
Review of outcome of pregnancy with history of previous caesarean section- predictors of safe labour
Objectives: To study the factors which affect outcome of trial of labor Materials and Methods: All pregnant women admitted to Goa Medical College, from October 2013 to June 2015, fulfilling the inclusion criteria were en...
Worm Obstruction In Children: Has Anything Changed Post Deworming Compaigns. A Tertiary Care Study.
A comprehensive and retrospective study was conducted at post graduate department of general surgery SKIMS Medical College, Bemina over a period of 5 years from February 2012 to March 2016 where about 70 patients (childr...
Giant Lipomas: A Report of Two Cases and Brief Review of Literature
Giant lipomas involving the extremities are rare tumors. These large masses grow slowly and produce symptoms due to their size, location and compression of adjacent structures. Here we present two interesting cases of gi...
A Comparative Study of Surgical Outcome in Acquired Nasolacrimal Duct obstruction after Conventional External DCR versus Endoscopic Endonasal DCR in a Tertiary Care Centre in Tripura
Dacryocystitis is the inflammation of the lacrimal sac. Spontaneous resolution does not occur without treatment. [1]Treatment of choice for this disorder is dacryocystorhinostomy(DCR). The previously published literature...
Importance of MDCT in Evaluation of Pancreatitis
Background and Objectives: MDCT is the modality of choice and can be employed for evaluation of both inflammatory and neoplastic conditions of the pancreas. MDCT provides high quality visualization of the parenchymal abn...