RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS QUANTITATIVE ESTIMATION OF DILOXANIDE FUROATE AND ORNIDAZOLE IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in tablets and validate as per ICH guidelines.Methods: The optimized method uses a reverse phase C18 column, ZODIAC (250 X 4.6 mm; 5μ), mobile phase consisting of mixed phosphate buffer (KH2PO4 and K2HPO4): acetonitrile in the proportion of 30:70 v/v. The mobile phase was set at a flow rate of 1.0 ml/min and the volume injected was 20μl for every injection. The detection wavelength was set at 279 nm. Results: The developed method resulted in Diloxanide furoate eluting at 4.293 min and Ornidazole at 3.34 min. Diloxanide furoate exhibited linearity in the range 90-210μg/ml, while Ornidazole exhibited linearity in the range 60-140μg/ml. The precision is exemplified by relative standard deviations of 0.97% for Diloxanide furoate and 0.3% for Ornidazole. Percentage Mean recoveries were found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) for Diloxanide furoate and Ornidazole were found to be 8.80µg/ml and 6.68µg/ml respectively, while limit of quantitiation (LOQ) for Diloxanide furoate and Ornidazole were found to be 26.6µg/ml and 20.25µg/ml respectively. Conclusion: A simple, accurate, precise, linear and rapid RP-HPLC method was developed for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in Amicline plus tablets and validated as per ICH guidelines. Hence it can be used for the routine analysis of Diloxanide furoate and Ornidazole in tablets in various pharmaceutical industries.
Authors and Affiliations
P. Sivaprasad, S. Yaswanth Kumar, A. Ashok Kumar
Keywords
Related Articles
- EP ID EP579125
- DOI -
- Views 89
- Downloads 0
EFFECT OF CAFFEINE IN EXPERIMENTAL MODEL OF RHEUMATOID ARTHRITIS IN RATS
Objective: Primary objective of this study was to evaluate anti-inflammatory effect of caffeine in complete Freund’s adjuvant model of rheumatoid arthritis. Secondary objective was to compare the topical anti-inflammat...
SYNTHESIS, CHARACTERISATION AND DNA PHOTOCLEAVAGE ACTIVITY OF NEW 2-(THIOXO/OXO) QUINOLINE-4,6-DIMETHYL PYRIMIDINYL HYDRAZONES
Objective: The main objective of present work is to synthesize, characterize and evaluate DNA photocleavage activity of hydrazones containing quinoline and pyrimidine rings.Methods: The syntheses of new 2-(Thioxo/Oxo)qui...
QUICK/SLOW BIPHASIC RELEASE OF A POORLY WATER SOLUBLE ANTIDIABETIC DRUG FROM BI-LAYER TABLETS
Objective: The objective of the present work is to develop a bi-layer tablet consisting of an Immediate Pulse Release [IPR] layer and a sustained release [SR] layer that can produce a distinct biphasic release having two...
NEUROPROTECTIVE EFFECT OF CITRULLUS LANATUS SEED EXTRACTS ON CEREBRAL ISCHEMIC REPERFUSION INJURY INDUCED COGNITIVE IMPAIRMENT AND OXIDATIVE STRESS
Objective: Oxidative stress appears to be an early event involved in the pathogenesis of Alzheimer's disease (AD). The present study was designed to investigate the neuroprotective effects of citrullus lanatus on bilater...
FORMULATION AND EVALUATION OF AN HERBAL ANTI-INFLAMMATORY GEL CONTAINING TRIGONELLA FOENUM GREACUM SEED EXTRACT
Objective: The present study was aimed to develop topical gel containing Trigonella foenum greacum (fenugreek) seed extract using carbopol-934, Hydroxypropyl methylcellulose K4M (HPMC K4M) as gelling agents and to invest...