RP-HPLC METHOD FOR DETERMINING THE LEVELS OF GUAIPHENESIN AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2016, Vol 5, Issue 7
Abstract
A simple, precise and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of guaiphenesin and phenylephrine in their tablet dosage form. The chromatographic conditions were standardized using an Inertsil column,C18 (150x4.6 ID) 5µm with UV detection at 277nm , and the mobile phase consisted of sodium dihydrogen phosphate buffer: Acetonitrile (30:70).The retention times of guaiphenesin and phenylephrine were About 3.497 min for Guaifenesin and 2.353min for Phenylephrine, respectively. The calibration curves were linear with correlation coefficients of 0.9987, 0.9988, 0.9981 and 0.9981 over a concentration range of 4.0–24.0 µg/ml for guaiphenesin, 5.0–30.0 µg/ml for phenylephrine, respectively. The proposed method has been validated according to the ICH guidelines and was successfully applied to estimate the levels of two drugs in a combined formulation with good accuracy and precision.
Authors and Affiliations
malladi babu, pavan kumar chadalawada, s. manohar babu
Keywords
Related Articles
- EP ID EP439375
- DOI -
- Views 142
- Downloads 0
A REVIEW ON TRADITIONAL LARVICIDAL PLANTS
Background: Mosquito are vectors of many serious diseases and spread rapidly. The destruction of mosquitos larvae is comparatively convenient than adult mosquitos and nymphs. Most of the countries using chemical to kill...
HEPATOPROTECTIVE ACTIVITY OF METHONOLIC EXTRACT OF HIBISCUS SABDARIFFA IN RATS
Objective: In the present study hepatoprotective activity was carried out in vivo models which include different. Treatment with aqueous leaf extract of H. sabdariffa caused significant increase in the serum levels. Meth...
COMPREHENSIVE NURSING CARE: CONCEPT ANALYSIS WITH THE EVOLUTIONARY APPROACH
Field and Target: Comprehensive nursing care has always been one of the main arguments of nursing profession. One simple definition of Holism is that whole of one thing is more than the sum of its components. The aim of...
FACTORS EFFECTIVE ON READINESS OF HOSPITALS AFFILIATED TO MEDICAL UNIVERSITY OF BOUSHEHR PROVINCE AGAINST ACCIDENTAL EVENTS IN 2017
Introduction: hospitals as one of the first centers of reception of injured at the time of accession of disasters should have required readiness. The present study was conducted with the aim of investigating factors effe...
MOLECULAR INTERACTION OF NATURAL BIFLAVONOIDS, AS COMPETITIVE ANTAGONISTS, WITH AROMATASE RELATED TO BREAST CANCER: AN IN SILICO APPROACH
Objective: Understanding the mechanism of inhibition exerted on aromatase by natural compounds is essential since such compounds may be helpful as a source of efficacious therapeutic agents for the management of postmeno...