RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF SEXAGLIPTIN AND PIOGLITAZONE IN TABLETS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 5
Abstract
A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Sexagliptin and Pioglitazone. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5m , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 260nm.Aceclofenac was used as an internal standard. The retention time of Sexagliptin, pioglitazone and aceclofenac was 2.48, 4.45 and 6.34 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation.This study aimed at developing and validating an HPLC method, being simple, accurate and selective, and the proposed method can be used for the estimation of these drugs in combined dosage forms.
Authors and Affiliations
M. Sarat , P. Murali krishna , C Rambabu
Keywords
Related Articles
- EP ID EP119689
- DOI -
- Views 94
- Downloads 0
NAVASADARA SATVAPATANA: AN INVITRO STUDY
Navasadara satvapatana is one of the unique contributions of the text Rasatarangini. A compound of Ammonium chloride is formed as a satva. It is indicated in puppusashotha, agnimandya, murcha etc. Like this it has many t...
EVALUATION OF PATIENT COUNSELING IN DIFFERENT HOSPITAL OF KARACHI, PAKISTAN; A NEGLECTED DOMAIN OF PHARMACY
Patient counseling is a critical component of dispensing process that provides information, advice and assistance to help patients using their medication properly. The objective of our study was to analyze whether patien...
OLD AND NEW EMERGING THEREPIES FOR MULTIPLE SCLEROSIS
Multiple sclerosis (MS) is an autoimmune disease that is characterized by central nervous system inflammation, demyelination, and axon loss. It’s affect up to two million people world wide and represents a prime cause of...
ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECT OF POLYGALA JAVANA DC ON ALLOXAN INDUCED DIABETIC RATS
The ethanol extract of Polygala javana whole plant (Family: Polygalaceae) was investigated for it antidiabetic and antihyperlipidaemic effect in Wistar Albino rats. Diabetes was induced in albino rats by administration o...
FORMULATION AND EVALUATION OF GASTRO RETENTIVE DRUG DELIVERY SYSTEM OF STAVUDINE
The present study was designed to formulate and evaluate hydro dynamically balanced Floating Drug Delivery Systems as controlled release modules, which prolongs the release rate of the drugs. Stavudine is an anti- retrov...