SACCHARIFICATION BY FUNGI AND ETHANOL PRODUCTION BY BACTERIA USING LIGNOCELLULOSIC MATERIALS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 5
Abstract
Lignocellulosic material is one of the most abundant, renewable and inexpensive energy resources for bioethanol production. These materials are mainly composed of three groups of polymers namely cellulose, hemicellulose and lignin. Cellulose and hemicellulose are sugar rich fractions of interest for use in fermentation processes such as ethanol production. Cellulase production by the different fungi like Trichoderma reesei (MTCC-4876), Phanerochaete chrysosporium (MTCC-787) and Aspergillus awamori (MTCC-6652) were studied using different substrates (rice straw, wheat straw and rice husk) by keeping the concentration constant at 5g/ 150 ml. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as inoculums. The maximum cellulase production and saccharification observed in the presence of combination of fungi with treated rice straw. Further Zymomonas mobilis bacteria was used for carrying out fermentation of sugars to ethanol production. Among the three raw materials studied the ethanol yield was observed to be the highest in rice straw ( 9.5 g/l ).
Authors and Affiliations
Ajeet Srivastava , Pushpa Agrawal
Keywords
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