SACCHARIFICATION OF ALKALINE TREATED RICE STRAW BY SUBSEQUENTLY HYDROLYSIS OF XYLANOLYTIC-CELLULOLYTIC ENZYMES FOR XYLOOLIGOSACCHARIDES AND GLUCOSE PRODUCTION
Journal Title: International Journal of Bio-Technology and Research (IJBTR) - Year 2017, Vol 7, Issue 3
Abstract
Rice straw (RS) is a biomass found abundantly in Thailand as a waste product from rice grain collections. This study aimed to produce xylooligosaccharides and glucose from RS. The xylan saccharification of aqueous ammonia treated RS was done by a xylanolytic enzyme cocktail (Xyn10E and GH43B6). The analysis of the final liberated product showed that a majority of xylobiose and minority of xylose and xylooligosaccharides were observed. The cellulose saccharification was subsequently conducted by a cellulolytic enzymes cocktail (Cel9R, Cbh9A, CglT). The synergistic action of the cellulolytic enzyme on alkaline treated and xylanolytic hydrolyzed RS liberated sole glucose as a final product. This is an alternative carrier for the total utilization of RS. The subsequent xylanolytic-cellulolytic enzyme hydrolysis of RS to its subunits leads to a potential feed stock for high value products production.
Authors and Affiliations
KANOK WONGRATPANYA, KHANOK RATANAKHANOKCHAI, JUNJARUS SERMSATHANASWADI, THIDARAT NIMCHUA, RATTIYA WAEONUKUL, PATTHRA PASON, CHAKRIT TACHAAPAIKOON, AKIHIKO KOSUGI
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