Screening and Isolation of Keratinase Producing Micro Organisms
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2017, Vol 5, Issue 5
Abstract
In present study 35 proteolytic isolates were isolated from various samples containing keratin rich sources from different areas in Kolhapur and Sangli region of Maharashtra state. These isolates were screened for production of proteolytic enzymes like caseinase,gelatinase by using milk agar and gelatin agar. All these isolates were gelatinase and caseinase positive.So, further used to check kertinolytic activity by studying feather degradation process.From all isolates,7 isolates named as I1, I6, P, M5-2,E2N,E4N,C9 shows keratinase activity.Out of which,isolate M5-2 show highest caseinase(10 mm zone),gelatinase(26 mm zone) activity along with 100% feather degradation(keratinase) activity.This keratinolytic isolate was further identified morphologically,biochemically and by 16s rRNA sequencing as Stenotrophomonas maltophilia YArck. The sequence is deposited at Gen Bank NCBI with gene accession no.KY941138.
Authors and Affiliations
Aishwarya B. Kurane, Yasmin. C. Attar
Keywords
Related Articles
- EP ID EP24111
- DOI -
- Views 298
- Downloads 12
Unified Visual Programming Environment
This paper briefly describes the process of programming which includes the creation, compilation and execution of programs to perform tasks. The Visual Programming Environment (VPE) provides visual approaches to program...
An Enhanced Event Based Data Transmission Approach with Updated Clustering in Wireless Communication Network
Wireless Sensor Network is a field which is growing rapidly for the purpose of communication. It includes the sensors distributed physically in order to sense the surrounding environment and collected data is transferre...
Introduction To Artificial intelligence
Artificial intelligence (AI), the ability of a digital computer or computer-controlled robot to perform tasks commonly associated with intelligent beings. The term is frequently applied to the project of developing syst...
Investigation of Biogas Flow Analysis in Mixing Chamber by Using CFD, Performance and Emission Characteristics of Single Cylinder SI Engine
In this work, Design and fabrication of mixing chamber for biogas supply to the engine and CFD analysis of the mixing Chamber. Biogas is used as fuel for SI engine by the help of pressure conversion kit, study of differ...
Vibration Analysis of Single Cylinder Diesel Engine Using Various Fuel Blends
Biodiesel as an environmentally friendly fuel which has the potential to provide comparable engine performance results. Biodiesel is a renewable fuel produced with the help of vegetable and seed oils. Sound and vibratio...