SCREENING FOR GMO IN FERMENTED SOY SAUCE
Journal Title: Genetics & Applications - Year 2017, Vol 1, Issue 1
Abstract
Soy sauce is worldwide popular condiment of Asian origin. With the advent of GM soybean production, soy sauce drew the interest of food safety control. Samples collected for inspection are generally of industrial grade soy sauce type, which is produced from hydrolyzed soybean and grain. Following the failure to perform RealTime PCR based GMO screening on a number of submitted samples we tested our screening system on soy sauce produced following traditional method based on fermentation. Four batches of soy sauce were produced and DNA extracted. DNA concentration ranged from 32.68 to 65.36 ng/μl. Amplification of taxon specific target was successful with rather high Ct ( > 30). Promoter P-35S sequence was not detected, but T-NOS was detected in three samples with values reaching or exceeding LOD of the method. The results show that it is possible to detect transgenic elements in traditionally produced soy sauce while DNA extraction from industrial grade soy sauce is not possible.
Authors and Affiliations
Anesa Ahatović, Edina Ljekperić, Mirza Nuhanović, Adaleta Durmić-Pašić
Keywords
Related Articles
- EP ID EP349647
- DOI 10.31383/ga.vol1iss1pp33-36
- Views 152
- Downloads 0
MUTATION ANALYSIS OF AUTOSOMAL STR LOCI COMMONLY USED IN PATERNITY TESTING IN BOSNIA AND HERZEGOVINA
Mutation analysis in forensic genetics and occurrence of mutations at short-tandem repeat (STR) loci, are very important in paternity testing and precise elucidation of obtained genetic profiles. To determine these locus...
MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA
The focus of this study was microsatellite diversity of crossbred horses raised in Bosnia and Herzegovina. Genomic DNA was extracted from blood samples of 20 individuals (KBA group – 7 individuals, crosses between Bosnia...
KNOWLEDGE OF ELEMENTARY GENETIC TERMINOLOGY AMONG HIGH SCHOOL STUDENTS IN BOSNIA AND HERZEGOVINA
Genetics, as a science of the future, is very important part of Biology teaching. Within educational system in Bosnia and Herzegovina it is studied in limited number of clasess with a minimum of practical excercises, in...
GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS
In order to assess the genetic purity of common buckwheat variety ‘Darja’ which is the most commonly produced variety of this crop in Bosnia and Herzegovina, 10 SSR markers have been used. Five samples have been collecte...
Special edition-book of abstracts
/