SCREENING OF ANTIBIOFILM AND ANTI-QUORUM SENSING POTENTIAL OF VITEX TRIFOLIA IN PSEUDOMONAS AERUGINOSA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 8
Abstract
Objective: In this present study the methanolic leaf extract of Vitex trifolia was assessed for its anti quorum sensing activity against acyl-homoserine lactone (AHL)-dependent virulence factors in Pseudomonas aeruginosa.Methods: In this study the methanolic leaf extract of Vitex trifolia was screened to control the virulence factors of Pseudomonas aeruginosa by antibacterial analysis, biofilm prevention assay, protease and elastase assay, pyocyanin production, EPS (Exopolysaccaride) assay, Swimming assay and Light microscopic analysis.Results: The methanolic leaf extract obtained from Vitex trifolia exhibited a concentration dependent (50-100 μg/ml) reduction in anti quorum sensing activity in Pseudomonas aeruginosa. The extract inhibited 69% of protease activity, 48% of elastase activity and 96% of exopolysaccharide production in Pseudomonas aeruginosa. The pigment pyocyanin production and swimming motility of the bacteria were also controlled.Conclusion: Thus in this study the methanolic leaf extract of Vitex trifolia was found to be potent against anti quorum sensing activity in Pseudomonas aeruginosa.Â
Authors and Affiliations
R. Nancy Immaculate Mary, N. Banu
Keywords
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