Screening Of Pectinolytic Fungi And Optimization Of Process Parameters Using Guava Peel Powder As Substrate Under Solid State Fermentation
Journal Title: International Journal of Engineering and Science Invention - Year 2018, Vol 7, Issue 10
Abstract
The disposal of waste from fruit processing industries is a major problem. Pectinase is widely used in the fruit industry for clarifying juices. So the waste from fruit industry can be utilized as a substrate for the production of pectinase by identifying new strains which can produce more amount of enzyme as the need of production of high amounts of pectinase is recommended. In this work, pectinases-producing filamentous fungi (P1) was isolated from the soil collected from fruit waste dump, with the aim of finding optimized condition for maximum production of pectinase. In the present study, the production of pectinase using Aspergillus niger NCIM 616 and isolate P1 was carried out under solid-state fermentation using guava waste powder as substrate. Both the fungi showed more or less similar pectinolytic activity. Among the isolates of G3 and P1, the P1 showed maximum pectionlytic activity. Different parameters optimization processes were investigated on SSF namely fermentation time (144 & 120 hr), temperature (300C), pH (8 & 6), moisture content (160% & 100%), inoculum volume (3ml & 3ml), inoculum age (120 & 144 hr) for Aspergillus niger and P1 isolate respectively.
Authors and Affiliations
Prathibha Bezawada, Karothi Jaya Raju
Keywords
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