SDS-PAGE Band Patterns of Partially Purified Peroxidase Isozymes Extracted from Telfairia Mosaic Virus (TeMV) Infected and HealthyP lant Leaves of Telfairia occide ntalis (Hook F.)
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 1
Abstract
This study was carried out to separate Telfairia occidentalis peroxidase isozymes extracted from leaves of Telfairia mosaic virus (TeMV) infected and healthy plants by SDS-PAGE and compare their banding patterns for difference. Telfairia occidentalis plants were grown under green house condition and arranged in a completely randomized design prior to inoculation. Randomly selected plants were inoculated with TeMV. Peroxidase isozymes extracted from healthy and infected plants were separated by SDS-PAGE, using 12.5% resolving gel buffered at pH 8.8 and 5% stacking gel in tris glycine buffered at pH 6.8. The isozymes profiles of healthy and infected plants were qualitatively analyzed for presence or absence of bands and quantitatively using Rf values. Analysis of the peroxidase isozyme profiles revealed that both healthy and infected T. occidentalis plants have seven identical bands each with Rf values 0.09, 0.30, 0.35, 0.43, 0.55, 0.68, 0.83 and molecular weights of 66.07, 36.31, 30.20, 23.44, 16.60, 11.75 and 7.08 respectively. The results suggest that SDS-PAGE of peroxidase enzymes cannot be used for detection of TeMV infection of T. occidentalis.
Authors and Affiliations
Mofunanya, A. A. J, Egbe, A. O.
Keywords
Related Articles
- EP ID EP393362
- DOI 10.9790/3008-1301037277
- Views 72
- Downloads 0
A Prospective Study on Prescription Pattern and Quality Of Life in Epilepsy Patients at a Private Corporate Hospital
A Prospective Study was conducted in Neurology Department on Prescription Pattern and Quality of Life in Epilepsy Patients at a Private Corporate Hospital in Coimbatore, India. A total of 214 cases were found, of which 8...
Occasional Observation of Acanthamoeba in the Pharynx. A Case Report
Acanthamoeba is an opportunistic protozoan "free-living" widely distributed in nature and is a potential pathogen for Man. It is responsible for pathologies inherent in the CNS, the cornea , the skin, especially in immun...
A Comparative Study of 80r and 240r Gamma Rays On Male Meiotic Chromosomes of Grasshopper Spathosternum Praciniferum
Adult healthy males of short horned Indian grasshopper, Spathosternum praciniferum were irradiated with gamma rays with a dose of 80r and 240r while the untreated specimens served as control. The testes material of treat...
Sero-Activity of Chikungunya Virus at the Kenyan Coast after the 2004 Epidemic
Chikungunya virus (CHIKV) is an alphavirus of Semliki Forest virus antigenic complex. Seroprevalence of CHIKVwas measured after the reported 2004CHIKV epidemic in Coastal Kenya and factors associated with seropositivity...
Crisis of Antibiotic Resistance -A Definite Ban on Healthcare
Anti-microbial resistance patterns can vary regionally and even among different hospitals within the same community. Infections are the most common reasons for patients to seek medical advice and for antibiotics to be pr...