SECOND DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RIFAMPICIN AND PIPERINE IN THEIR COMBINED DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 4
Abstract
The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective Second derivative spectrophotometric zero crossing point method for the simultaneous determination of Rifampicin and Piperine in combined capsule dosage form. The utility of Second derivative data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The Second order derivative absorption at 341 nm (zero cross point for Piperine) was used for Rifampicin and 241 nm (zero cross point for Rifampicin) was used for Piperine. The linearity was obtained in the concentration range of 10-60 μg/ml for Rifampicin and 2-20 μg/ml for Piperine. The method was successfully applied to pharmaceutical dosage form because no interference from the capsule excipients was found. The suitability of these methods for the quantitative determination of Rifampicin and Piperine was proved by validation. The proposed methods were found to be simple and sensitive for the routine quality control application of Rifampicin and Piperine in pharmaceutical capsule dosage form. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Jenil Khamar , Satish Patel
Keywords
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