Sensitivity and specificity of 99m Tc-UBI29-41 and 67 Ga-Citrate scintigraphy imaging to discriminate infection lesion induced by Staphylococcus aureus and sterile inflammation lesion induced by Carrageenan in foot’s rat
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 11
Abstract
This study was launched to evaluate the efficacy and efficiency of 99m Tc-UBI29-41scintigraphy imaging to visualize the infection foci induced by staphylococcus aureus and inflammation lesions induced by carrageenan in the foot’s rat in comparison with 67Ga-Citrate radioisotope scintigraphy imaging. The labeling and quality control of 99m Tc-UBI29-41have been performed according to the manufacturer’s instructions. A total number thirty six adult, male NMRI rats were chosen. The animals were randomly divided into two equal group’s .One group for 99m Tc-UBI29-41 scintigraphy imaging and the other group for 67 Ga-Citrate scintigraphy imaging respectively. Every group subdivided into two groups equally. Septic lesion was induced by Staphylococcus aureus due to inoculation of bacteria suspension in the foot’s rat in one group. The aseptic inflammation lesion was induced by Carrageenan in the foot’s rat in the other group. The 99m Tc-UBI29-41 and 67 Ga-Citrate radiotracer scintigraphy imaging studies have been performed to evaluate the sensitivity and specificity 99m Tc-UBI29-41radiopharmaceutical for preferentially diagnosis between infection and sterile inflammation lesions. The images have been shown the uptake 67 Ga at the infection and inflammation sites. The labeling of UBI by technetium can provide images with good quality and a shorter investigation time in comparison to 67 Ga radioisotope imaging. The infection foci could be visualized by 99m Tc-UBI29- 41scintigraphy imaging due to selective bonding UBI 29-41 to the negatively charged groups present on the microbial membrane due to electrostatic interaction. The inflammation sites have been observed by non-specific uptake of 99m Tc-UBI29-41. Both scintigraphy imaging studies have not demonstrated preferentially diagnosis septic and aseptic inflammation lesions. The sensitivity, specificity and positive predictive value of both scintigraphy imaging techniques were 100%, 50% and 50% respectively. In spite of high sensitive of the 99m TcUBI 29-41 scintigraphy imaging to localize the lesions, but it could not demonstrate to discriminate between septic and aseptic inflammation lesions. The other modalities must be considered for interpretation of images has obtained by 99m Tc-UBI 29-41 scintigraphy imaging study.
Authors and Affiliations
A. Doroudi , M. Erfani , , K. Damavandi Kamali 1 , S. M. Saadati 3 , F. Ahmadi 3 ,A. Kiasat 3 ,M. J. Khodayar 1 , H. Meghdadi 4
Keywords
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